Effect of infection with murine recombinant retroviruses containing the v-src oncogene on interleukin 2- and interleukin 3-dependent growth states.
Effect of infection with murine recombinant retroviruses containing the v-src oncogene on interleukin 2- and interleukin 3-dependent growth states.
复制标题
含有 v-src 癌基因的鼠重组逆转录病毒感染对白细胞介素 2 和白细胞介素 3 依赖性生长状态的影响。
DOI：
10.4049/jimmunol.139.1.123
复制
发表时间：
1987
影响因子：
4.4
通讯作者：
S. Gillis
中科院分区：
文献类型：
--
作者：
J. Watson;M. Eszes;R. Overell;P. Conlon;M. Widmer;S. Gillis
克隆的鼠白细胞介素 3 (IL 3) 依赖性细胞系 FD.C/1、32Dc1-23 和 KP3 均可转换为白细胞介素 2 (IL 2) 依赖性生长状态。复制缺陷型逆转录病毒载体已用于将 v-src 癌基因引入这些维持 IL 3 或 IL 2 依赖性生长状态的细胞系中的每一个中。这些维持在IL 3依赖性生长状态的细胞系在用v-src感染后转变为不依赖淋巴因子的生长。这些相同的细胞维持IL 2依赖性生长状态并感染v-src，维持严格的淋巴因子生长依赖性。另一种克隆的鼠 IL 3 依赖性细胞系 GM 可以转换为粒细胞-巨噬细胞集落刺激因子 (GM-CSF) 依赖性生长状态。当感染 v-src 时，维持为 IL 3 或 GM-CSF 依赖性细胞的 GM 细胞很容易转变为不依赖淋巴因子的生长状态。这些实验表明，通过 IL 3 和 IL 2 特异性受体进行信号转导的生化机制存在差异，或者与细胞向 IL 2 依赖性生长状态转换相关的发育过程影响 v-src 基因产物的表达。这些细胞系不仅为分析调节细胞生长的生化途径提供了新方法，而且还为分析癌基因表达的控制提供了新方法。
The cloned murine interleukin 3 (IL 3)-dependent cell lines FD.C/1, 32Dc1-23, and KP3 can each be switched to interleukin 2 (IL 2)-dependent growth states. Replication-defective retroviral vectors have been used to introduce the v-src oncogene into each of these cell lines maintained in either an IL 3- or an IL 2-dependent growth state. These cell lines maintained in an IL 3-dependent growth state were converted to lymphokine-independent growth after infection with v-src. These same cells maintained in an IL 2-dependent growth state and infected with v-src maintained strict lymphokine dependence for growth. Another cloned murine IL 3-dependent cell line, GM, can be switched to a granulocyte-macrophage colony-stimulating factor (GM-CSF)-dependent growth state. GM cells maintained as IL 3- or GM-CSF-dependent cells readily converted to a lymphokine-independent growth state when infected with v-src. These experiments indicate that either there exist differences in the biochemical mechanisms of signal transduction through the IL 3- and IL 2-specific receptors, or developmental processes associated with the switching of cells to an IL 2-dependent growth state influence expression of the v-src gene product. These cell lines offer new ways not only for analyzing biochemical pathways that regulate cell growth, but also for analyzing the control of oncogene expression.