Exposure to 900 MHz electromagnetic fields activates the mkp-1/ERK pathway and causes blood-brain barrier damage and cognitive impairment in rats
Exposure to 900 MHz electromagnetic fields activates the mkp-1/ERK pathway and causes blood-brain barrier damage and cognitive impairment in rats
复制标题
暴露于 900 MHz 电磁场会激活 mkp-1/ERK 通路，导致大鼠血脑屏障损伤和认知障碍
DOI：
10.1016/j.brainres.2015.01.019
复制
发表时间：
2015-03-19
期刊：
影响因子：
2.9
通讯作者：
Zhu, Gang
中科院分区：
文献类型：
--
作者：
Tang, Jun;Zhang, Yuan;Zhu, Gang
随着移动的电话用户数量的迅速增加，由移动的电话发射的电磁场辐射的潜在不利影响已成为严重关注的问题。本研究首次证明了暴露于900 MHz电磁场（EMF）的大鼠的血脑屏障和认知变化，旨在阐明这些变化背后的潜在分子途径。共108只雄性Sprague-Dawley大鼠暴露于900 MHz、1 mW/cm（2）EMF或假手术（未暴露）14或28天（每天3小时）。比能量吸收率（SAR）在0.016（全身）和2 W/kg（头部局部）之间变化。此外，采用Morris水迷宫实验检测大鼠空间记忆能力的测定。通过观察海马和皮质超微结构的变化来观察形态学变化，并使用Evans Blue测定来评估血脑屏障（BBB）的损伤。免疫组化检测血红素氧合酶-1（HO-1）阳性神经元和白蛋白外渗。Western blot检测HO-1、磷酸化ERK及上游调节因子mkp-1的表达。我们发现，频率的交叉平台和花费在目标象限的时间的百分比在暴露于电磁场28天的大鼠低于暴露于电磁场14天的大鼠和未暴露的大鼠。此外，28天的电磁场暴露诱导细胞水肿和神经元细胞器变性的大鼠。此外，在海马和皮质中观察到BBB通透性受损，这导致白蛋白和HO-1外渗。因此，我们首次发现，电磁场暴露28天诱导mkp-1的表达，导致ERK去磷酸化。这些结果表明，暴露于900 MHz电磁场辐射28天可以显着损害大鼠的空间记忆和损伤BBB通透性通过激活mkp-1/ERK通路。(C)2015 Elsevier B. V.版权所有。
With the rapid increase in the number of mobile phone users, the potential adverse effects of the electromagnetic field radiation emitted by a mobile phone has become a serious concern. This study demonstrated, for the first time, the blood-brain barrier and cognitive changes in rats exposed to 900 MHz electromagnetic field (EMF) and aims to elucidate the potential molecular pathway underlying these changes. A total of 108 male Sprague-Dawley rats were exposed to a 900 MHz, 1 mW/cm(2) EMF or sham (unexposed) for 14 or 28 days (3 h per day). The specific energy absorption rate (SAR) varied between 0.016 (whole body) and 2 W/kg (locally in the head). In addition, the Morris water maze test was used to examine spatial memory performance determination. Morphological changes were investigated by examining ultrastructural changes in the hippocampus and cortex, and the Evans Blue assay was used to assess blood brain barrier (BBB) damage. Immunostaining was performed to identify heme oxygenase-1 (HO-1)-positive neurons and albumin extravasation detection. Western blot was used to determine HO-1 expression, phosphorylated ERK expression and the upstream mediator, mkp-1 expression. We found that the frequency of crossing platforms and the percentage of time spent in the target quadrant were lower in rats exposed to EMF for 28 days than in rats exposed to EMF for 14 days and unexposed rats. Moreover, 28 days of EMF exposure induced cellular edema and neuronal cell organelle degeneration in the rat. In addition, damaged BBB permeability, which resulted in albumin and HO-1 extravasation were observed in the hippocampus and cortex. Thus, for the first time, we found that EMF exposure for 28 days induced the expression of mkp-1, resulting in ERK dephosphorylation. Taken together, these results demonstrated that exposure to 900 MHz EMF radiation for 28 days can significantly impair spatial memory and damage BBB permeability in rat by activating the mkp-1/ERK pathway. (C) 2015 Elsevier B.V. All rights reserved.