Cytogenetics of Alismataceae and Limnocharitaceae: CMA/DAPI banding and 45S rDNA sites
Cytogenetics of Alismataceae and Limnocharitaceae: CMA/DAPI banding and 45S rDNA sites
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DOI：
10.1007/s00606-010-0300-7
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发表时间：
2010-05
影响因子：
1.9
通讯作者：
L. Feitoza;M. Martins;A. Castro;L. Felix;Reginaldo de Carvalho
中科院分区：
文献类型：
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作者：
L. Feitoza;M. Martins;A. Castro;L. Felix;Reginaldo de Carvalho
种属于泽泻科（Echinodorus）和Limnocharitaceae（HydrocleysandLimnocharis）家庭进行了分析，带与CMA/DAPI荧光染料，C/CMA/DAPI带，原位杂交（FISH）与探针，识别45 S rDNA。所有棘属的种均为2n= 22，但仅E.在7对染色体上观察到DAPI+端粒带。双峰核型和GC丰富的异染色质优选位于两个较小的近端着丝粒对，通常对应于45 S rDNA的位点数。在两种湖沼石首鱼（2n= 20）中观察到相似的带型，但在45 SrDNA上两种石首鱼的带型不同。lafresti只显示了两个位点：Hydrocleys nymphoides和H. martii的染色体数目为2n= 16，但与随体相关的富含GC的异染色质的位置在染色体类型之间存在差异。在这项工作中，讨论了这些模式的细胞分类学意义，并与文献中以前的数据进行了关联。
Species belonging to the Alismataceae (Echinodorus) and Limnocharitaceae (HydrocleysandLimnocharis) families were analysed by banding with CMA/DAPI fluorochromes, C/CMA/DAPI banding, and in situ hybridization (FISH) with probes that recognise 45S rDNA. All species ofEchinodoruspresented 2n= 22, but only inE. lanceolatuswere DAPI+ telomeric bands in seven chromosome pairs observed. A bimodal karyotype and GC-rich heterochromatin preferably located in two smaller acrocentric pairs that generally corresponded to the number of sites of 45S rDNA. A similar pattern of bands was observed in bothLimnocharisspecies (2n= 20), but the two differed with respect to 45S rDNA, withL. laforestiishowing only two sites.Hydrocleys nymphoidesandH. martiihad a chromosome number of 2n= 16, but the position of the GC-rich heterochromatin associated with the satellite differed among chromosomal types. In this work, the cytotaxonomic implications of these patterns are discussed and correlated with previous data from the literature.