Quantitative Analysis of the Corneal Collagen Distribution after In Vivo Cross-Linking with Second Harmonic Microscopy
Quantitative Analysis of the Corneal Collagen Distribution after In Vivo Cross-Linking with Second Harmonic Microscopy
复制标题
DOI：
10.1155/2019/3860498
复制
发表时间：
2019-01-01
影响因子：
--
通讯作者：
Carmen Martinez-Garcia, M.
中科院分区：
文献类型：
--
作者：
Bueno, Juan M.;Avila, Francisco J.;Carmen Martinez-Garcia, M.
角膜交联（CXL）是一种能够改变角膜生物力学并稳定圆锥角膜进展的外科手术。尽管众所周知 CXL 会导致角膜胶原分布发生变化，但这些仍然是一个讨论的话题。在这里，我们使用二次谐波发生 (SHG) 成像显微镜和结构张量 (ST) 定量比较体内常规 CXL 治疗 4 周后两种动物模型之间的角膜基质结构。还通过组织学切片分析了愈合阶段和基质恢复情况。结果表明，CXL 效果取决于角膜胶原蛋白的初始排列。虽然治疗增加了初始组织水平较低的角膜的秩序，但呈现相当规则模式的角膜几乎不受影响。组织学样本显示前基质和中基质中有活跃的角膜细胞，这意味着恢复仍在进行中。 SHG成像和ST的结合能够客观地区分CXL治疗后胶原排列所遭受的变化，其有效性取决于角膜基质内胶原纤维的初始组织。
Corneal cross-linking (CXL) is a surgical procedure able to modify corneal biomechanics and stabilize keratoconus progression. Although it is known that CXL produces changes in corneal collagen distribution, these are still a topic of discussion. Here we quantitatively compare the corneal stroma architecture between two animal models four weeks after in vivo conventional CXL treatment, with second harmonic generation (SHG) imaging microscopy and the structure tensor (ST). The healing stage and the stroma recovery were also analyzed by means of histological sections. Results show that the CXL effects depend on the initial arrangement of the corneal collagen. While the treatment increases the order in corneas with a low level of initial organization, corneas presenting a fairly regular pattern are hardly affected. Histological samples showed active keratocytes in anterior and middle stroma, what means that the recovery is still in progress. The combination of SHG imaging and the ST is able to objectively discriminate the changes suffered by the collagen arrangement after the CXL treatment, whose effectiveness depends on the initial organization of the collagen fibers within the corneal stroma.