Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles
Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles
复制标题
将身体赋予鬼魂：将分子定位在它们发挥生物学作用的地方
DOI：
10.4081/ejh.2024.3950
复制
发表时间：
2024
期刊：
影响因子：
--
通讯作者：
C. Redi
中科院分区：
文献类型：
--
作者：
Ernesto Capanna;C. Redi
本文回顾了我们多年来在Rivista di Istochimica Normale e Patologica，Basic and Applied Histochemistry和European Journal of Histochemistry - EJH上发表的研究目标。在一系列论文中，我们首次发表了精子细胞分化过程的一些基本细胞化学特征。这是一个概念和实践的先决条件，在原位定量评价精子DNA含量。我们发现，当比较精子与体细胞DNA含量时，预期的1：2比例之间的差异（精子DNA含量总是远远低于理论值）是由于福尔根反应引起的盐酸处理引起的DNA损失。在福尔根反应的各个步骤中发生的特定损失的知识使我们能够在基因组大小研究中严格地使用它来强调：-染色体衍生的低生育力中的精子非整倍性; -哺乳动物基因组大小的广泛变异范围; -白蚁是蟑螂（经过几十年的讨论）。此外，在一篇关于人类卵母细胞的开创性论文中，我们（通过透射电子显微镜）显示了与卵母细胞成熟停滞相关的特定染色质和细胞质组织（两者对进一步的胚胎发育至关重要），这一数据与人类和兽医生殖中未满足的治疗需求非常相关。
This paper reviews some of the goals of our investigations published over the years on Rivista di Istochimica Normale e Patologica, Basic and Applied Histochemistry, and the European Journal of Histochemistry - EJH. In a series of papers, we published some of the basic cytochemical features of the sperm cytodifferentiation process for the first time. This was a conceptual and practical prerequisite to the in situ quantitative evaluation of sperm DNA content. We showed that the discrepancy between the expected 1:2 ratio when comparing sperm versus somatic cell DNA content (sperm DNA content is always far low from the theoretical value) is due to DNA losses caused by the hydrochloric treatment entailed by the Feulgen reaction. The knowledge of the specific losses that occur during the various steps of the Feulgen reaction has allowed us to use it critically in Genome Size studies to highlight: - sperm aneuploidy in chromosomally derived subfertility; - the broad variability range of Mammalian genome sizes; - that termites are roaches (after decades of discussion on this topic). In addition, in a seminal paper on human oocytes, we showed (by transmission electron microscopy) a specific chromatin and cytoplasmic organization (both essential for further embryo development) linked to oocyte maturation arrest, a datum quite relevant to treating unmet therapeutic needs in human and veterinary reproduction.