Anti-inflammatory Activity of Mycelial Extracts from Medicinal Mushrooms
Anti-inflammatory Activity of Mycelial Extracts from Medicinal Mushrooms
复制标题
药用蘑菇菌丝体提取物的抗炎活性
DOI：
10.1615/intjmedmushrooms.v16.i4.20
复制
发表时间：
2014-01-01
影响因子：
1.2
通讯作者：
Xu, Zheng-Hong
中科院分区：
文献类型：
--
作者：
Geng, Yan;Zhu, Shuiling;Xu, Zheng-Hong
几千年来，药用蘑菇一直是传统中草药的重要成分，它们可以预防各种与健康有关的疾病。其抗炎活性的成分尚未得到充分研究。本研究考察了5种市售药用蘑菇——中国头孢菌、虫草、猴头菌、灵芝和蜜环菌——菌丝体的正己烷、氯仿、乙酸乙酯和甲醇提取物的抗炎活性。采用MU比色法测定不同提取物的细胞毒作用。通过抑制小鼠巨噬细胞样细胞系RAW264.7细胞产生脂多糖（LPS）诱导的一氧化氮（NO）来评估其抗炎活性。在20个提取物中，分别有中华青霉、mortierella和灵芝的正己烷、氯仿、乙酸乙酯和甲醇提取物；蛇尾草和蜜蜂草的氯仿提取物；和无害浓度的蜜蜂乙酸乙酯提取物(
Medicinal mushrooms have been essential components of traditional Chinese herbal medicines for thousands of years, and they protect against diverse health-related conditions. The components responsible for their anti-inflammatory activity have yet to be fully studied. This study investigates the anti-inflammatory activity of n-hexane, chloroform, ethyl acetate, and methanol extracts of mycelia in submerged culture from 5 commercially available medicinal mushrooms, namely Cephalosporium sinensis, Cordyceps mortierella, Hericium erinaceus, Ganoderma lucidum, and Armillaria mellea. MU colorimetric assay was applied to measure the cytotoxic effects of different extracts. Their anti-inflammatory activities were evaluated via inhibition against production of lipopolysaccharide (LPS)-induced nitric oxide (NO) in murine macrophage-like cell line RAW264.7 cells. Of the 20 extracts, n-hexane, chloroform, ethyl acetate, and methanol extracts from C. sinensis, C. mortierella, and G. lucidum; chloroform extracts from H. erinaceus and A. mellea; and ethyl acetate extracts from A. mellea at nontoxic concentrations (