Keratin gene expression in simian virus 40-transformed human keratinocytes.
Keratin gene expression in simian virus 40-transformed human keratinocytes.
复制标题
猿猴病毒 40 转化的人角质形成细胞中角蛋白基因的表达。
DOI：
10.1073/pnas.82.24.8498
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发表时间：
1985
影响因子：
11.1
通讯作者：
Defendi,V
中科院分区：
文献类型：
--
作者：
Morris,A;Steinberg,ML;Defendi,V
该实验室以前的报告表明，培养的猿猴病毒40 （SV40）转化的人角质形成细胞表达简单上皮细胞特征的角蛋白，而这些蛋白在未转化的同类细胞中没有发现。在这项研究中，我们通过体外翻译和RNA转移印迹分析表明，角蛋白合成的改变反映了特定角蛋白mrna丰度的变化。与未感染的培养角质形成细胞相比，sv40转化的角质形成细胞的58-、56-、52-、50-、48-和46-kDa角蛋白的转录物丰度降低，但单纯上皮细胞典型的52-、45-和40-kDa角蛋白的转录物表达水平显著。sv40转化的细胞也表达一种48kda角蛋白的mRNA，这是sv40转化的角质形成细胞所特有的。用角蛋白特异性cdna作为探针对角蛋白基因组进行分析表明，角蛋白转录的变化与角蛋白基因组的总体重排无关。这些结果表明，分析培养的角质形成细胞的病毒转化为研究调节角蛋白基因表达的机制提供了一种新的方法。
Previous reports from this laboratory indicate that cultured simian virus 40 (SV40)-transformed human keratinocytes express keratin proteins characteristic of simple epithelia that are not found in their untransformed counterparts. In this study we show by in vitro translation and RNA transfer blot analysis that the altered keratin synthesis reflects changes in the abundance of specific keratin mRNAs. SV40-transformed keratinocytes have a reduced abundance of transcripts for 58-, 56-, 52-, 50-, 48-, and 46-kDa keratin species, compared with uninfected cultured keratinocytes, but express significant levels of transcripts for 52-, 45-, and 40-kDa keratins, typical of simple epithelia. The SV40-transformed cells also express mRNA for a 48-kDa keratin that is unique to SV40-transformed keratinocytes. Analysis of the keratin genome with keratin-specific cDNAs as probes indicates that the changes in keratin transcription are not correlated with gross rearrangements of the keratin genome. These results suggest that analysis of viral transformation of cultured keratinocytes affords a novel approach to study mechanisms regulating keratin gene expression.