Genomic Distribution of a Long Terminal Repeat (LTR) Sabrina-like Retrotransposon in Triticeae Species
Genomic Distribution of a Long Terminal Repeat (LTR) Sabrina-like Retrotransposon in Triticeae Species
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小麦科物种长末端重复 (LTR) Sabrina 样反转录转座子的基因组分布
DOI：
10.1556/crc.37.2009.3.5
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发表时间：
2009-09-01
影响因子：
1.6
通讯作者：
Ren, Z. L.
中科院分区：
文献类型：
--
作者：
Liu, C.;Yang, Z. J.;Ren, Z. L.
长末端重复序列（LTR）的染色体分布对于理解植物染色体结构、基因组组织和进化以及为染色体工程提供有用的染色体标志是重要的。本研究的目的是研究首次从短芒簇毛麦（Dasypyrum breviaristatum）中分离到的Sabrina类LTR pDbH 12（V-b基因组）在小麦族植物中的基因组分布及其与基因组进化和染色体鉴定的关系。荧光原位杂交（FISH）分析表明，pDbH 12基因存在于簇毛麦（Dasypyrum，V基因组）和大麦（Hordeum，H基因组）中，杂交信号覆盖了整条染色体。然而，克隆pDbH 12不与黑麦、小麦、Lophopyrum、假芒草、山羊草、冰草和Escherichus的基因组杂交。中间偃麦草显示出与pDbH 12杂交的14条染色体。序列FISH鉴定这些染色体属于J（s）基因组。SCAR标记和斑点杂交结果均支持FISH结果，综合分析结果表明，Sabrina类LTR反转录转座子的扩增是参与物种形成过程的重要因素。克隆pDbH 12可作为小麦异源渐渗系中V或V-b、H和J（s）染色体组染色质追踪的细胞遗传学标记。
Knowledge of the chromosomal distribution of long terminal repeats (LTR) is important for understanding plant chromosome structure, genomic organization and evolution, as well as providing chromosomal landmarks that are useful for chromosome engineering. The aim of this study is to investigate the genomic distribution of Sabrina-like LTR pDbH12, which was first isolated from Dasypyrum breviaristatum (V-b genome), on Triticeae species in relation to the genomic evolution and chromosome identification. Fluorescence in situ hybridization ( FISH) analysis showed that pDbH12 is present on Dasypyrum ( V genome) and Hordeum ( H genome) species with the hybridized signals covering the entire chromosomes. However, clone pDbH12 did not hybridize to the genomes of Secale, Triticum, Lophopyrum, Pseduoroengeria, Aegilops, Agropyron desertorum and Elymus. Thinopyrum intermedium displayed fourteen chromosomes that hybridized with pDbH12. Sequential FISH identified these chromosomes as belonging to the J(s) genome. Results from sequence characterized amplified region ( SCAR) marker and dot blot both support the FISH results, and the integrative results suggest that amplification of Sabrina-like LTR retrotransposons is an important factor which involved in the speciation process. Clone pDbH12 could serve as a cytogenetic marker for tracing chromatin from V or V-b, H and J(s) genomes in wheat-alien introgression lines.