Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)
Physiologic and biochemical measurements and response to noxious stimulation at various concentrations of MS-222 in Koi (Cyprinus carpio)
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DOI：
10.1111/j.1467-2995.2012.00739.x
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发表时间：
2013-01-01
影响因子：
1.7
通讯作者：
Paul-Murphy, Joanne
中科院分区：
文献类型：
--
作者：
Stockman, Jonathan;Weber, Ernest Scott P., III;Paul-Murphy, Joanne
目的探讨5种浓度MS-222对鲤的生理效应及对有害刺激的反应。研究设计前瞻性实验研究。21条性别不详的健康成年锦鲤，平均体重450 +/- SD 120 g。方法将不同浓度的MS-222（50、70、110、150和190 mg L-1）随机暴露于同一麻醉过程中。在标准化诱导期后，记录MS-222各浓度的心率（HR）（通过多普勒）和眼率（OpR）等重要功能。对两种有害刺激（以止血钳的形式施加在尾巴和嘴唇上）的反应进行评估，并抽血测量生化和血气值。结果随着MS-222浓度的增加，大鼠唇部（p = 0.0027）和尾部（p < 0.0001）对有害刺激的反应均有所降低。除乳酸浓度与麻醉时间（r = 0.31, p < 0.001）和取样前夹鱼或放血次数（r = 0.23, p < 0.001）呈弱相关外，生化指标不受MS-222浓度的影响。随着麻醉浓度的增加，眼率降低，HR不受影响。结论和临床意义我们的研究结果表明，MS-222浓度的增加与刺激反应的降低和OpR的降低有关。这可能有助于建立在鱼类中使用MS-222的麻醉方案，并支持在可变MS-222浓度下使用超最大压力刺激来硬骨鱼，作为未来研究的模型。
Objective To evaluate the physiological effect and response to noxious stimulation at five concentrations of MS-222 in koi (Cyprinus carpio).Study design Prospective experimental study. Animals Twenty-one healthy adult unknown sex koi fish weighing mean 450 +/- SD 120 g.Methods Each fish was exposed to five different concentrations of MS-222 (50, 70, 110, 150 and 190 mg L-1) in a random sequence during the same anaesthetic event. For each concentration of MS-222, vital functions such as heart rate (HR) (via Doppler) and opercular rate (OpR) were recorded after a standardized induction period. Response to two noxious stimuli in the form of haemostat clamp pressure applied on the tail and the lip was evaluated, and blood was drawn to measure biochemical and blood gas values.Results Decrease in response to noxious stimulation with an increase of MS-222 concentration both for the lip (p = 0.0027) and the tail (p < 0.0001) stimulus was observed. Biochemical values were unaffected by the concentration of MS-222 with the exception of lactate concentration which was weakly correlated with the duration of anaesthesia (r = 0.31, p < 0.001) and the number of times the fish was clamped or bled prior to sampling (r = 0.23, p < 0.001). Opercular rate decreased with the increase in anaesthetic concentration, and HR was not affected.Conclusions and clinical relevance Our results indicated a decrease in response to stimulus and a decrease in OpR that were associated with increased concentrations of MS-222. This may assist in establishing anaesthetic protocols using MS-222 in fish and supports the use of supramaximal pressure stimuli to teleost fish under variable MS-222 concentrations as a model for future studies.