Recent Progress of Thioester Method1
Recent Progress of Thioester Method1
复制标题
硫酯法最新进展1
DOI：
10.1016/s1005-9040(06)60091-3
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发表时间：
2006
影响因子：
3.1
通讯作者：
T. Kawakami
中科院分区：
文献类型：
--
作者：
Aimoto Saburo;Kenta Teruya;K. Hasegawa;Takeshi Sato;K. Akaji;T. Kawakami
3 部分得到日本文部科学省科学研究 Grand2in2A id 的支持（编号：10179103 和 12780440）。 3 3 信件应寄给谁。电子邮件：okada@pharm。神户学院。交流。 jp 引言 位点特异性修饰肽的可用性对于生物化学和生物物理研究至关重要。生物学方法，例如使用细菌表达，是有用的。然而，它们并不总是适用于具有位点特异性修饰的肽的合成。化学方法可以成为生物方法的可行替代方案。通过化学和生物手段获得的肽作为构建模块，为合成多种多肽提供了巨大的潜力。 1991年，Hojo和A imoto [1, 2]报道了一种以S 2 烷基肽硫酯（肽硫酯）为结构单元的多肽合成方法。此后，硫酯法得到了改进[3-5]。该方法现在可用于合成各种类型的多肽（图1），包括磷酸2 6]和糖蛋白7]。
3 Supported partially by a Grand2in2A id for Scientific Research from the M inistry of Education, Science, Sports and Culture of Japan (Nos. 10179103 and 12780440). 3 3 To whom correspondence should be addressed. E2mail: okada@ pharm. kobegakuin. ac. jp In troduction The availability of site2specifically modified pep2 tides is of vital importance for biochem ical and biophys2 ical studies. B iological methods, such as exp ression using bacteria, are useful. They are, however, not al2 ways app licable to the synthesis of pep tides with site2 specific modifications. Chem ical methods can be viable alternatives to those biological app roaches. Pep tides obtained by chem ical and biological means, as building blocks, offer a great potential to the synthesis of a wide variety of polypep tides. In 1991, Hojo and A imoto [1, 2] reported a method for polypep tide synthesis in which S 2alkyl pep tide thioesters (pep tide thioesters) were used as building blocks. Since then, imp rovements have been made to the thioester method [3—5]. The method is now useful in the synthesis of various types of polypep tides (Fig. 1) including phospho2 6] and glycop roteins 7].