The effect of EGF application in gel form on histamine content of experimentally induced wound in mice
The effect of EGF application in gel form on histamine content of experimentally induced wound in mice
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DOI：
10.1007/s00726-004-0103-7
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发表时间：
2004-12-01
期刊：
影响因子：
3.5
通讯作者：
Özogul, C
中科院分区：
文献类型：
--
作者：
Babül, A;Gönül, B;Özogul, C
影响创伤愈合的因素是复杂的，至今仍不清楚。在这些因素中，表皮生长因子（EGF）和组胺通过增加再上皮化和修复组织强度，通过增强胶原蛋白沉积到伤口部位，具有有益的效果。本研究通过组织学观察、伤口抗张强度测定、胶原成熟度测定和组胺含量测定，观察EGF对小鼠实验性创伤组织中组胺含量的影响，并对创伤愈合指标进行评价。将32只小鼠分为对照组和EGF治疗组。对照包括三个亚组：未处理（n = 5）、应用0.9%NaCl（n = 5）和应用凝胶（n = 5）。实验组用两种形式的EGF处理; EGF，在0.9%NaCl中的溶液形式（n = 5）和在卡波姆940中的0.2%w/w的凝胶形式（n = 7）。对这些表格之间的差异进行了评价。通过应用两种形式的EGF对实验诱导的伤口愈合进行15天的评估，发现通过从生物粘附聚合物持续释放的EGF的凝胶形式比可溶形式对伤口愈合更有效，通过加速再上皮化和增加伤口拉伸强度。伤口的抗张强度表明了修复和胶原蛋白成熟的速度。已经观察到，当生理盐水和卡波姆940暴露于没有EGF的切口时，会导致组织组胺含量显著增加。根据本研究的结果，发现EGF凝胶剂型治疗可降低组胺含量，因此防止异常胶原形成对伤口愈合具有有益作用。
The factors participating to the wound healing are complex and still obscure. Among these factors, epidermal growth factor (EGF) and histamine by increasing reepithelization and reparation tissue strength via enhancing collagen deposition to the wound site have a beneficial effect. This study was performed to investigate the effect of EGF dosage forms on the histamine content of the experimentally induced wound and some wound healing criters in the mice.Histological investigation of reepithelization, wound tensile strength for healing and collagen maturation, and histamine levels were assessed in the present study. Thirty two mice were divided into control, and EGF treated groups. Controls included three subgroups; untreated (n = 5), 0.9% NaCl applied ( n = 5), and gel applied ( n = 5). Experimental groups were treated with two forms of EGF; EGF, solution form in 0.9% NaCl ( n = 5) and the gel form in 0.2% w/w in carbopol 940 ( n = 7). The discrepancy between these forms were evaluated. This evaluation was done by the application of two forms of EGF for 15 days on experimentally induced wound healing.Gel form of EGF by sustained release from bioadhesive polymer is found to be more effective than the soluble form, on the healing of the wound, by acceleration of reepithelization and increment of wound tensile strength. The tensile strength of the wound indicates the rate of repair and collagen maturation. It has been observed that when physiological saline and carbopol 940 exposed to incision without EGF causes a significant increase in tissue histamine content.According to the results of the present investigation; the histamine content is found to be decreased by EGF gel dosage form treatment, therefore preventing abnormal collagen formation has a beneficial effect on wound healing.