Peyer's patch innate lymphoid cells regulate commensal bacteria expansion
Peyer's patch innate lymphoid cells regulate commensal bacteria expansion
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DOI：
10.1016/j.imlet.2015.03.002
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发表时间：
2015-05-01
影响因子：
4.4
通讯作者：
Kobata, Tetsuji
中科院分区：
文献类型：
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作者：
Hashiguchi, Masaaki;Kashiwakura, Yuji;Kobata, Tetsuji
先天淋巴细胞 (ILC) 促进肠粘膜中共生细菌的解剖学遏制。然而，ILC 调节细菌定位到特定区域的机制仍不清楚。在这里，我们展示了派尔氏集结（PP）ILC 在没有外源刺激的情况下强劲地产生 IL-22 和 IFN-γ。对小鼠进行抗生素治疗可减少 PP 中的 IL-22(+) 和 IFN-gamma(+) 细胞。体外阻断 IL-2 和 IL-23 信号传导可降低 IL-22 和 IFN-γ 的产生。 PP ILC 诱导肠上皮细胞中抗菌蛋白 RegIII beta 和 RegIII gamma 的 mRNA 表达。此外，体内 ILC（而不是 T 细胞）的消耗改变了细菌组成，并允许细菌在 PP 中增殖。总的来说，我们的结果表明 ILC 调节 PP 中共生细菌的扩增。 (C) 2015 年欧洲免疫学会联合会。由 Elsevier B.V. 出版。保留所有权利。
Anatomical containment of commensal bacteria in the intestinal mucosa is promoted by innate lymphoid cells (ILCs). However, the mechanism by which ILCs regulate bacterial localization to specific regions remains unknown. Here we show that Peyer's patch (PP) ILCs robustly produce IL-22 and IFN-gamma in the absence of exogenous stimuli. Antibiotic treatment of mice decreased both IL-22(+) and IFN-gamma(+) cells in PPs. Blockade of both IL-2 and IL-23 signaling in vitro lowered IL-22 and IFN-gamma production. PP ILCs induced mRNA expression of the antibacterial proteins RegIII beta and RegIII gamma in intestinal epithelial cells. Furthermore, in vivo depletion of ILCs rather than T cells altered bacterial composition and allowed bacterial proliferation in PPs. Collectively, our results show that ILCs regulate the expansion of commensal bacteria in PPs. (C) 2015 European Federation of Immunological Societies. Published by Elsevier B.V. All rights reserved.