Platelet growth factors suppress ex vivo expansion and enhance differentiation of umbilical cord blood CD133+ stem cells to megakaryocyte progenitor cells
Platelet growth factors suppress ex vivo expansion and enhance differentiation of umbilical cord blood CD133+ stem cells to megakaryocyte progenitor cells
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DOI：
10.3109/08977194.2010.504722
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发表时间：
2010-12-01
期刊：
影响因子：
1.8
通讯作者：
Gharehbaghian, Ahmad
中科院分区：
文献类型：
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作者：
Aghideh, Ali Noroozi;Kheirandish, Maryam;Gharehbaghian, Ahmad
背景与目的脐血(UCB)是一种丰富的造血细胞来源，本研究首次探讨了不同浓度的血小板生长因子和细胞因子组合(CC)对脐血CD133(+)干细胞扩增和分化为巨核系祖细胞的影响。材料与方法采用磁性细胞分选法分离脐血CD133(+)细胞，并将其与含有白细胞介素3、6、干细胞因子和血小板生成素的CC联合培养，用单核细胞计数和流式细胞仪检测细胞扩增和分化。结果无论是富含血小板的血浆还是培养上清液，均可诱导脐血CD133(+)细胞向巨核细胞分化培养第1天加入，培养7天后，CD133(+)细胞的扩增受到明显抑制(p<；CD41、CD61和CD42b的表达均高于对照组(p<；005)。结论CC可抑制脐血CD133(+)细胞的体外扩增，促进其向巨核系祖细胞分化，并呈剂量和时间依赖关系。
Background and objectives Umbilical cord blood (UCB) is a rich source of hematopoietic cells Here, for the first time, we surveyed the effects of different concentrations of platelet growth factors and cytokine cocktail (CC) on the expansion and differentiation of UCB CD133(+) stem cells into megakaryocyte progenitorsMaterials and methods UCB CD133(+) cells were separated by magnetic cell sorting and cultured in different concentrations of platelet growth factors m combination with a CC containing interleukins 3 and 6, stem cell factor, and thrombopoietin Cell expansion and differentiation were assessed using mononuclear cell count and flow cytometryResults The results show that either activated platelet-rich plasma or the platelet supernatant, when added in the first day of culture, significantly suppress the expansion of CD133(+) cells after 7 days in culture (p < 0 05) By contrast, the expression of CD41, CD61, and CD42b markers m the presence of all platelet growth factors increased compared with that of the control (p < 0 05)Conclusion Taken together, platelet growth factors m the presence of CC suppress ex vivo expansion of UCB CD133(+) cells and enhance their differentiation into megakaryocytic progenitor cells in a dose- and time-dependent manner