Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems.
Rapid anterograde axonal transport of the cellular prion glycoprotein in the peripheral and central nervous systems.
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发表时间：
1994-05
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通讯作者：
D. R. Borchelt;V. Koliatsos;Michael Guarnieri;Carlos A. Pardo;S. Sisodia;Donald L. Price
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作者：
D. R. Borchelt;V. Koliatsos;Michael Guarnieri;Carlos A. Pardo;S. Sisodia;Donald L. Price
在朊病毒疾病中，大量存在于神经元中的细胞朊病毒蛋白（PrPc）在翻译后转化为淀粉样蛋白形成的瘙痒朊病毒蛋白（PrPSc），在白质束和神经末梢积累。通过在成年大鼠L4、L5背根神经节和内嗅皮层注射[35S]蛋氨酸，并追踪放射性标记的PrPc的运动，研究了PrPc在神经元中的体内运输。在这两种模式下，标记的33-35-kDa PrPc在4小时内被运输到远端轴突和神经末梢，与快速成分中的蛋白质共同作用。使用这些方法的未来研究可能使我们能够确定PrPc是否在轴突转运过程中转化为PrpSc，以及PrpSc是否在朊病毒疾病动物中转运。
In prion diseases, the cellular prion protein (PrPc), abundant in neurons, is converted posttranslationally into an amyloid-forming scrapie prion protein (PrPSc), which accumulates in white matter tracts and nerve terminals. The trafficking of PrPc in neurons was investigated in vivo by injecting [35S]methionine into the L4 and L5 dorsal root ganglia and the entorhinal cortices of adult rats and by tracing the movement of radiolabeled PrPc. In both paradigms, labeled 33-35-kDa PrPc was transported, within 4 h, to distal axons and nerve terminals cofractionating with proteins in the fast component. Future studies using these methods may allow us to determine whether PrPc is converted into PrpSc during axonal transport and whether PrPSc is transported in animals with prion diseases.