Strongyloides ratti:: Chemokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro
Strongyloides ratti:: Chemokinesis of glycolytic enzyme- and lectin-treated third-stage infective larvae in vitro
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DOI：
10.1016/j.parint.2005.03.001
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发表时间：
2005-06-01
影响因子：
1.9
通讯作者：
Tada, I
中科院分区：
文献类型：
--
作者：
Tobata-Kudo, H;Kudo, H;Tada, I
在体外琼脂糖追踪实验中，啮齿动物寄生线虫黑圆线虫（Strongyloides ratti）感染性第三期幼虫在氯化钠（NaCl）梯度下表现出两种类型的趋化作用。在不利环境条件下的定向回避行为是一致的，在有利环境条件下的定向回避行为是减少的。我们通过分析回避行为来检验糖酵解酶和凝集素治疗的效果。L-聚焦脱氢酶、透明质酸酶、-葡萄糖苷酶、-甘露糖苷酶、-半乳糖苷酶、豆豆蛋白A、小麦胚芽凝集素和大豆凝集素对趋化运动表现出抑制或增强作用。我们还通过扫描电镜确认了美国两栖动物在口腔前端以外的位置，并通过凝集素组织化学证实了豆豆蛋白a的结合位点存在于两栖动物附近。ratti Us两栖动物体内的碳水化合物可能在感知环境信号中起着重要的化学传感器作用。2005爱思唯尔爱尔兰有限公司版权所有。
The infective third-stage larvae (Us) of Strongyloides ratti, a parasitic nematode in rodents, showed two types of chemokinesis on a gradient of sodium chloride (NaCl) in an in vitro agarose tracking assay. The types were a consistent directional avoidance behavior under unfavorable environmental conditions and a reduced avoidance behavior under favorable conditions. We examined the effects of treatments with glycolytic enzymes and lectins by analyzing the avoidance behavior. L-Fucose dehydrogenase, hyaluronidase, beta-glucosidase, alpha-mannosidase, beta-galactosidase, concanavalin A, wheat germ agglutinin and soybean agglutinin exhibited inhibitory or enhancive effects on chemokinesis. We also confirmed the sites of the amphids of Us aside from the mouth at the anterior end by scanning electron microscopy, and that concanavalin A-binding sites existed in the vicinity of the amphids using lectin-histochemistry. The carbohydrate moieties in the amphids of S. ratti Us may play an important role as chemosensors in perceiving environmental cues. (c) 2005 Elsevier Ireland Ltd. All rights reserved.