Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe.
Six git genes encode a glucose-induced adenylate cyclase activation pathway in the fission yeast Schizosaccharomyces pombe.
复制标题
DOI：
--
复制
发表时间：
1993-08
影响因子：
4
通讯作者：
S. Byrne;C. S. Hoffman
中科院分区：
文献类型：
--
作者：
S. Byrne;C. S. Hoffman
一个重要的真核生物信号转导途径涉及调节效应酶腺苷酸环化酶，其产生第二信使cAMP。先前的遗传分析表明，葡萄糖阻遏粟酒裂殖酵母fbp1基因的转录需要由git2基因编码的腺苷酸环化酶的功能。由于git 2和另外6个git基因的突变被外源性cAMP抑制，因此这些“上游”git基因被认为是产生葡萄糖诱导的cAMP信号的作用。我们在这里报告，在野生型和各种突变体S。在暴露于葡萄糖之前和之后的粟酒裂殖酵母细胞表明情况正是如此。数据表明cAMP信号是由腺苷酸环化酶的激活引起的。因此，这些“上游”git基因似乎编码葡萄糖诱导的腺苷酸环化酶激活途径。cAMP在git6基因（其作用于腺苷酸环化酶下游）中携带突变的菌株上的测定表明，git6可能起反馈调节腺苷酸环化酶活性的作用。因此，git6可能编码cAMP依赖性蛋白激酶。
An important eukaryotic signal transduction pathway involves the regulation of the effector enzyme adenylate cyclase, which produces the second messenger, cAMP. Previous genetic analyses demonstrated that glucose repression of transcription of the Schizosaccharomyces pombe fbp1 gene requires the function of adenylate cyclase, encoded by the git2 gene. As mutations in git2 and in six additional git genes are suppressed by exogenous cAMP, these 'upstream' git genes were proposed to act to produce a glucose-induced cAMP signal. We report here that assays of cAMP levels in wild-type and various mutant S. pombe cells, before and after exposure to glucose, show that this is the case. The data suggest that the cAMP signal results from the activation of adenylate cyclase. Therefore these 'upstream' git genes appear to encode a glucose-induced adenylate cyclase activation pathway. Assays of cAMP on a strain carrying a mutation in the git6 gene, which acts downstream of adenylate cyclase, indicate that git6 may function to feedback regulate adenylate cyclase activity. Thus git6 may encode a cAMP-dependent protein kinase.