INVIVO AND INVITRO FATE OF FENVALERATE IN HOUSE-FLIES
INVIVO AND INVITRO FATE OF FENVALERATE IN HOUSE-FLIES
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DOI：
10.1016/0048-3575(90)90047-6
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发表时间：
1990-10-01
影响因子：
4.7
通讯作者：
PRYOR, NW
中科院分区：
文献类型：
--
作者：
BULL, DL;PRYOR, NW
对拟除虫菊酯抗性家蝇(R)和易感家蝇(S)的调查提供了确凿的证据，表明实验室选择的R菌株对氰戊菊酯的高水平交叉抗性是受该毒物代谢降解能力增强的影响。在存在或不存在混合功能氧化酶（mfo）抑制剂胡椒酰丁醇（PBO）或酯酶抑制剂S，S，S-三丁基硫代酸酯（DEF）的情况下，S和R家蝇匀浆制剂对[14C]氰戊酸酯体外代谢的比较研究表明，mfo活性的升高是代谢增强的主要组成部分，并且酯酶的增强与R虫匀浆制剂的微粒体和细胞质亚细胞部分相关。体内研究表明，共给药PBO或DEF对局部应用[14C]氰戊菊酯在R虫体内的吸收和代谢的影响表明，两种增效剂都减缓了毒物渗透角质层的速度；PBO显著降低了氰戊菊酯的代谢降解，而DEF对代谢的影响则不太确定。
Investigations of pyrethroid resistant (R) and susceptible (S) house flies (Musca domestica L.) provided conclusive evidence that a high level of cross-resistance to fenvalerate in the laboratory-selected R strain was influenced by an enhanced capability for metabolic degradation of the toxicant. Comparative studies of the in vitro metabolism of [14C]fenvalerate by homogenate preparations of S and R house flies, in the presence or absence of the mixed function oxidase (mfo) inhibitor piperonyl butoxide (PBO) or the esterase inhibior S,S,S-tributylphosphorothioate (DEF), indicated that elevation of mfo activity was a major component of enhanced metabolism and that there also was enhancement of esterase associated with both the microsomal and cytosolic subcellular fractions of homogenate preparations of R insects. In vivo studies of the effects of coadministered PBO or DEF on the absorption and metabolism of topically applied [14C]fenvalerate in R insects demonstrated that both synergists slowed the rate at which the toxicant penetrated the cuticle; PBO significantly reduced the metabolic degradation of fenvalerate, but effects of DEF on metabolism were less conclusive.