THE TOTAL MARROW MASS OF THE MOUSE - A SIMPLIFIED METHOD OF MEASUREMENT
THE TOTAL MARROW MASS OF THE MOUSE - A SIMPLIFIED METHOD OF MEASUREMENT
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DOI：
10.1002/ajh.2830160309
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发表时间：
1984-01-01
影响因子：
12.8
通讯作者：
BOGGS, DR
中科院分区：
文献类型：
--
作者：
BOGGS, DR
小鼠长骨中有核细胞的总数可以有一定的准确性。因此，在这个物种中，骨髓细胞的值可以用每个骨骼的细胞总数来表示，这个数字比在大多数其他物种的研究中用浓度来表示的值更有意义。如果骨骼中包含的骨髓在骨骼中所占的百分比是已知的，那么数值可以表示为“每只小鼠”(总骨髓质量)--这个数值比每个骨骼的数值更有意义。之前，小鼠的总骨髓质量是根据每个肱骨或股骨的有核细胞以及该骨骼中包含的总骨髓的百分比来计算的。然而，这一百分比是基于对整个骨骼的相当繁琐的解剖，并确定了每块骨头所吸收的59Fe的量。在本研究中，小鼠被注射59Fe，剥皮，并剔除内脏。然后，身体被煮熟，所有的骨头都被解剖出来，或者简单地切成包含各种骨骼或骨组的碎片。用这两种技术测量的不同骨骼或骨组吸收的59Fe的百分比实际上是相同的。在注射59Fe后的4至18小时内，不同骨骼之间的百分比分布相当稳定，在3个月或12个月大的小鼠中也是如此。这种简化的技术使总骨髓质量的测量在小鼠造血研究中成为一项实用的补充。
The total number of nucleated cells in the long bone of a mouse can be determined with some accuracy. Thus, in this species, values for marrow cells can be expressed as a total cell count per bone, a more meaningful number than values expressed as concentration as is done in most studies of other species. If the percent of total marrow in the skeleton that is contained in that bone is known, values can be expressed as “per mouse” (total marrow mass)–‐a still more meaningful value than values per bone. The total marrow mass of mice has been calculated previously on the basis of nucleated cells per humerus or per femur and the percent of the total marrow contained in that bone. However, that percent was based on rather tedious dissection of the entire skeleton and determining the amount of59Fe that had been taken up by each bone. In the present study, mice were injected with59Fe, skinned, and eviscerated. The carcass was then either cooked and all bones dissected out or simply cut into pieces containing various bones or bone groups. The percent of59Fe taken up by various bones or bone groups as measured by the two techniques was virtually identical. The percent distribution between various bones was found to be fairly constant between 4 and 18 h after59Fe injection and the same in mice aged 3 or 12 months. This simplified technique makes the measurement of total marrow mass a practical addition in studies of murine hematopoiesis.