Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae
Catalase T and Cu,Zn-superoxide dismutase in the acetic acid-induced programmed cell death in Saccharomyces cerevisiae
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DOI：
10.1016/j.febslet.2007.12.007
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发表时间：
2008-01-01
期刊：
影响因子：
3.5
通讯作者：
Passarella, Salvatore
中科院分区：
文献类型：
--
作者：
Guaragnella, Nicoletta;Antonacci, Lucia;Passarella, Salvatore
为了探讨过氧化氢酶和超氧化物歧化酶在醋酸(AA)诱导的酵母细胞程序性死亡(AA-PCD)中的作用，我们比较了酵母细胞(C-Y)和单独过表达过氧化氢酶T(CTT1-Y)和铜，锌超氧化物歧化酶(SOD-Y)的细胞在AA处理200分钟后的细胞存活率、过氧化氢(H(2)O(2))水平和酶活性。CTT1-Y不存在AA-PCD，H(2)O(2)水平低于C-Y，过表达的过氧化氢酶活性随时间降低。在SOD-Y，AA-PCD加重，H(2)O(2)水平高，SOD活性早期升高，在向AA-PCD发展的过程中保持不变，但过氧化氢酶活性显著降低。(C)2007年欧洲生化学会联合会。爱思唯尔出版，版权所有。
To investigate the role of catalase and superoxide dismutase (SOD) in the acetic acid (AA) induced yeast programmed cell death (AA-PCD), we compared Saccharomyces cerevisiae cells (C-Y) and cells individually over-expressing catalase T (CTT1-Y) and Cu,Zu-SOD (SOD1-Y) with respect to cell survival, hydrogen peroxide (H(2)O(2)) levels and enzyme activity as measured up to 200 min after AA treatment. AA-PCD does not occur in CTT1-Y, where H(2)O(2) levels were lower than in C-Y and the over-expressed catalase activity decreased with time. In SOD1-Y, AA-PCD was exacerbated; high H(2)O(2) levels were found, SOD activity increased early, remaining constant en route to AA-PCD, but catalase activity was strongly reduced. (C) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.