Evaluation of the effect of governmental control of human exposure to two phthalates in Japan using a urinary biomarker approach.
Evaluation of the effect of governmental control of human exposure to two phthalates in Japan using a urinary biomarker approach.
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DOI：
10.1016/j.ijheh.2005.02.004
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发表时间：
2005-07
影响因子：
6
通讯作者：
H. Itoh;Kikuo Yoshida;S. Masunaga
中科院分区：
文献类型：
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作者：
H. Itoh;Kikuo Yoshida;S. Masunaga
在日本，某些邻苯二甲酸盐的使用受到了监管。我们使用分析性生物标记物方法分析了这些措施的有效性。我们采用酶去共轭、离线固相萃取和高效液相-电喷雾串联质谱仪测定了36名受试者尿样中邻苯二甲酸单丁酯(MBP)和邻苯二甲酸单乙基己酯(MEHP)两种邻苯二甲酸单丁酯代谢物。尿MBP和MEHP值分别为1.80~280g/L和0.76~25μg/100g。35例成人尿样中邻苯二甲酸二丁酯和邻苯二甲酸二乙基己酯的估计日摄入量范围分别为0.22~4.5mgg/kg/d和0.37~7.3mgg/kg/d。这些值低于相应的TDI值。在将这些值与日本以前关于DEHP的暴露评估数据进行比较后，可以看出DEHP的摄入量在1998年至2001年期间有所下降。这项研究不包括儿童，因此结果可能只适用于成年人，而不适用于整个日本人口。即便如此，一个特定地理区域的少数人也不能被视为代表日本成年人口。除MEHP外，DEHP的次生代谢产物是更适合的生物标志物，应在未来进行检测。
In Japan, the use of certain phthalates has been regulated. We analyzed the effectiveness of these measures using an analytical biomarker approach. We measured two phthalate metabolites, mono-n-butyl phthalate (MBP) and mono-2-ethylhexyl phthalate (MEHP) in urine samples from 36 participants using enzymatic deconjugation, offline solid phase extraction, and HPLC-ESI-MS/MS. From the levels measured and individual values of creatinine excretion rate the daily intake was determined and compared to each corresponding tolerable daily intake (TDI). The levels of urinary MBP and MEHP were <1.8–280 and 0.76–25μg/l, respectively. The ranges of the estimated daily intake of di-n-butyl phthalate (DBP) and di-2-ethylhexyl phthalate (DEHP) from 35 adult urine samples were 0.22–4.5 and 0.37–7.3μg/kg/day, respectively. These values were lower than the corresponding TDIs. After comparing these values to previous exposure assessment data on DEHP in Japan, it could be seen that the DEHP intake dropped over the period from 1998 to 2001. Children were not covered in the present study, so the results may only be applicable to adults, not to the Japanese population at large. Even so, the small number of people in one specific geographic area cannot be considered representative of the adult Japanese population. In addition to MEHP, the secondary metabolites of DEHP, which are more suitable biomarkers should be measured in the future.