Effect of amphotericin B on membranes: a spin probe study.
Effect of amphotericin B on membranes: a spin probe study.
复制标题
两性霉素 B 对膜的影响：旋转探针研究。
作者：
Y. Aracava;I. Smith;S. Schreier
检查了多烯抗生素两性霉素 B 对嵌入模型膜中的脂质探针的电子顺磁共振谱的影响。当抗生素添加到水相中时，没有发生光谱效应。然而，当在膜制备过程中加入抗生素时，光谱参数的变化表明出现了新相。光谱变化不一定证实之前提出的膜中两性霉素 B 的孔模型。使用分隔水和膜的旋转探针，可以观察到两性霉素 B 和探针之间的相互作用。这种相互作用不会发生在膜中，而是发生在水相中、探针和聚集的抗生素之间。一些涉及抗生素的平衡似乎是缓慢实现的。
The effect of the polyene antibiotic amphotericin B on the electron paramagnetic resonance spectra of lipid probes intercalated in model membranes was examined. When the antibiotic was added to the aqueous phase, no spectral effects occurred. However, when the antibiotic was incorporated during membrane preparation, changes in spectral parameters suggested the appearance of a new phase. The spectral changes do not necessarily corroborate the pore models proposed previously for amphotericin B in membranes. With a spin probe that partitions between water and membrane, an interaction between the amphotericin B and probe is observed. This interaction does not occur in the membrane, but in the aqueous phase, between the probe and the aggregated antibiotic. Some of the equilibria involving the antibiotic appear to be achieved slowly.