Evaluation of alternative rice planthopper control by the combined action of oil-formulated Metarhizium anisopliae and low-rate buprofezin
Evaluation of alternative rice planthopper control by the combined action of oil-formulated Metarhizium anisopliae and low-rate buprofezin
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油剂金龟子绿僵菌与低倍噻嗪酮联用替代防治稻飞虱效果评价
DOI：
10.1002/ps.2026
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发表时间：
2011-01-01
影响因子：
4.1
通讯作者：
Chen, Jue-Qi
中科院分区：
文献类型：
--
作者：
Jin, Shao-Feng;Feng, Ming-Guang;Chen, Jue-Qi
背景：褐飞虱对常用杀虫剂的高抗药性是褐飞虱防治面临的挑战。一种替代的控制策略的基础上的真菌和化学试剂的联合应用已经evaluated.RESULTS：三个梯度孢子浓度的油配制金龟子绿僵菌（Metschnikoff）Sorokin（Ma 456）喷洒到三龄幼虫在5个生物测定，包括低噻嗪酮率0，10，20，30和40 μ g mL（-1）分别。在25 ℃和14：10 h光照：黑暗光周期下1周后，真菌LC 50从无噻嗪酮生物测定中的386分生孢子mm（-2）降至最高化学速率下的40。噻嗪酮（LC 50：1647，486和233 μ g/m（L-1）在第2天至第4天）在低施用率对真菌病杀死的尸体的真菌生长没有显著影响。发现真菌感染导致BPH成人生殖潜力降低81%。在两个40天的田间试验中，两种候选真菌每两周喷洒一次，（Ma 456和Ma576）在1.5 × 10（13）分生孢子ha（-1）时的抑制率提高到80-83%，并且通过将30.8g噻嗪酮ha（-1）掺入真菌喷雾剂中而提高到80-83%。真菌和化学药剂的联合应用是一种很有前途的替代策略。(C)2010年化学工业协会
BACKGROUND: High resistance of brown planthopper (BPH) Nilaparvata lugens Stal to common insecticides is a challenge for control of the pest. An alternative control strategy based on the combined application of fungal and chemical agents has been evaluated.RESULTS: Three gradient spore concentrations of oil-formulated Metarhizium anisopliae (Metschnikoff) Sorokin (Ma456) were sprayed onto third-instar nymphs in five bioassays comprising the low buprofezin rates of 0, 10, 20, 30 and 40 mu g mL(-1) respectively. Fungal LC50 after 1 week at 25 degrees C and 14 : 10 h light : dark photoperiod decreased from 386 conidia mm(-2) in the buprofezin-free bioassay to 40 at the highest chemical rate. Buprofezin (LC50: 1647, 486 and 233 mu g m(L-1) on days 2 to 4) had no significant effect on the fungal outgrowths of mycosis-killed cadavers at the low application rates. The fungal infection was found to cause 81% reduction in reproductive potential of BPH adults. In two 40 day field trials, significant planthopper (mainly BPH) control (54-60%) was achieved by biweekly sprays of two fungal candidates (Ma456 and Ma576) at 1.5 x 10(13) conidia ha(-1) and elevated to 80-83% by incorporating 30.8 g buprofezin ha(-1) into the fungal sprays.CONCLUSION: The combined application of the fungal and chemical agents is a promising alternative strategy for BPH control. (C) 2010 Society of Chemical Industry