Biomimetic Mineralized Hydroxyapatite Nanofiber-Incorporated Methacrylated Gelatin Hydrogel with Improved Mechanical and Osteoinductive Performances for Bone Regeneration.
Biomimetic Mineralized Hydroxyapatite Nanofiber-Incorporated Methacrylated Gelatin Hydrogel with Improved Mechanical and Osteoinductive Performances for Bone Regeneration.
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DOI：
10.2147/ijn.s354127
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发表时间：
2022
影响因子：
8
通讯作者：
Song J
中科院分区：
文献类型：
--
作者：
Wang H;Hu B;Li H;Feng G;Pan S;Chen Z;Li B;Song J
甲基丙烯酸酐改性明胶（GelMA）水凝胶表现出许多有益的生物学特性，并被广泛研究用于骨组织再生。然而，由于其在机械强度、成骨因子和矿物质离子等方面的不足，限制了其在骨缺损修复中的应用。在GelMA中加入无机填料以改善其力学性能和骨再生能力一直是研究热点之一。本工作制备了羟基磷灰石纳米纤维（HANFs），并在模拟体液中矿化，使其成分和结构更接近天然骨磷灰石，然后将不同量的矿化HANFs（m-HANFs）掺入到GelMA水凝胶中，形成m-HANFs/GelMA复合水凝胶。对m-HANFs/GelMA复合材料的理化性质、生物相容性和骨再生能力进行了体内、体外实验。结果表明，高长径比的m-HANFs表面粗糙多孔，表面包覆有类骨磷灰石晶体。仿生m-HANFs的引入改善了GelMA的生物相容性、力学性能、溶胀性能、降解性能和骨再生性能。但是，复合水凝胶性能的改善并没有随着m-HANFs添加量的增加而持续增加，15 m-HANFs/GelMA组在所有组中表现出最好的溶胀和降解性能以及最好的体内骨修复效果。仿生m-HANFs/GelMA复合水凝胶为骨组织工程提供了一种新的选择，但m-HANFs与GelMA的比例优化对骨修复效果的影响尚需进一步研究。
Methacrylic anhydride-modified gelatin (GelMA) hydrogels exhibit many beneficial biological features and are widely studied for bone tissue regeneration. However, deficiencies in the mechanical strength, osteogenic factors and mineral ions limit their application in bone defect regeneration. Incorporation of inorganic fillers into GelMA to improve its mechanical properties and bone regenerative ability has been one of the research hotspots. In this work, hydroxyapatite nanofibers (HANFs) were prepared and mineralized in a simulated body fluid to make their components and structure more similar to those of natural bone apatite, and then different amounts of mineralized HANFs (m-HANFs) were incorporated into the GelMA hydrogel to form m-HANFs/GelMA composite hydrogels. The physicochemical properties, biocompatibility and bone regenerative ability of m-HANFs/GelMA were determined in vitro and in vivo. The results indicated that m-HANFs with high aspect ratio presented rough and porous surfaces coated with bone-like apatite crystals. The incorporation of biomimetic m-HANFs improved the biocompatibility, mechanical, swelling, degradation and bone regenerative performances of GelMA. However, the improvement in the performance of the composite hydrogel did not continuously increase as the amount of added m-HANFs increased, and the 15m-HANFs/GelMA group exhibited the best swelling and degradation performances and the best bone repair effect in vivo among all the groups. The biomimetic m-HANFs/GelMA composite hydrogel can provide a novel option for bone tissue engineering in the future; however, it needs further investigations to optimize the proportions of m-HANFs and GelMA for improving the bone repair effect.