RAPID-DETERMINATION OF INTRAEPITHELIAL RESISTANCE BARRIERS BY ALTERNATING-CURRENT SPECTROSCOPY .1. EXPERIMENTAL PROCEDURES
RAPID-DETERMINATION OF INTRAEPITHELIAL RESISTANCE BARRIERS BY ALTERNATING-CURRENT SPECTROSCOPY .1. EXPERIMENTAL PROCEDURES
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DOI：
10.1007/bf00583942
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发表时间：
1984-01-01
影响因子：
4.5
通讯作者：
FROMTER, E
中科院分区：
文献类型：
--
作者：
KOTTRA, G;FROMTER, E
建立了一种用交流光谱仪同时快速测定渗漏上皮细胞紧密连接电阻、细胞横向间隙电阻、顶侧和基底外侧细胞膜的电阻和电容的方法。本文描述了实验过程。多频正弦波电流同时作用于整个组织，跨上皮和细胞内电压响应用屏蔽微电极记录，数字化并存储在计算机中。使用快速傅立叶变换技术，然后根据电压和电流波形计算出频率相关的跨上皮阻抗和表观基底膜阻抗。通过将适当的模型电路与数据进行拟合，可以推导出上面列出的各个电阻。分析的频率范围在2.5赫兹-12.5千赫之间。最小测量时间约为1-2个S。
A method was developed to determine rapidly and simultaneously the resistance of the tight junctions, the resistance of the lateral intercellular space and the resistances and capacitances of the apical and basolateral cell membrane in leaky epithelia by alternating current spectroscopy. This paper describes the experimental procedures. Multi-frequency sine-wave currents are simultaneously applied across the tissue and the transepithelial and intracellular voltage responses are recorded with shielded microelectrodes, digitized and stored in a computer. Using Fast Fourier Transform techniques the frequency-dependent transepithelial impedance and an apparent basal cell membrane impedance are then calculated from the voltage and current waveform. By fitting appropriate model circuits to the data the above listed individual resistances can be deduced. The analysis covers the frequency range between 2.5 Hz-12.5 kHz. The minimal measuring time is in the order of 1-2 s.