Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers
Definition of the Beijing/W lineage of Mycobacterium tuberculosis on the basis of genetic markers
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DOI：
10.1128/jcm.42.9.4040-4049.2004
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发表时间：
2004-09-01
影响因子：
9.4
通讯作者：
van Soolingen, D
中科院分区：
文献类型：
--
作者：
Kremer, K;Glynn, JR;van Soolingen, D
结核分枝杆菌北京基因型菌株在亚洲国家和前苏联境内高度流行。它们在世界其他地区的报道越来越多，并且经常与结核病爆发和耐药性相关。北京基因型毒株，包括W毒株，其特征在于高度相似的多拷贝IS6110限制性片段长度多态性（RFLP）模式、同向重复区域中间隔区1至34的删除（北京spoligo型）以及在基因组dnaA-dnaN位点中插入IS6110。本研究评估了这三个遗传标记识别北京谱系成员的适用性和可比性。在荷兰发现的代表 IS6110 RFLP 基因型的 1,020 株结核分枝杆菌分离株中，两个分支的菌株具有北京谱系特征的 spoligo 型。选择一组 19 个北京参考 RFLP 模式从荷兰数据库中检索所有北京菌株。这些参考模式在包含 1,084 个毒株的国际数据库中识别北京毒株（通过 spoligotyping 定义）的灵敏度为 98.1%，特异性为 99.7%。参考模式的有用性还通过其他两个欧洲国家的大型 DNA 指纹数据库以及美国发现的 W 谱系的鉴定菌株进行了评估。如本工作所述，属于北京/W谱系的结核分枝杆菌菌株鉴定的标准化定义将有助于进一步研究这种广泛传播的结核分枝杆菌基因型家族的传播和特征。
Mycobacterium tuberculosis Beijing genotype strains are highly prevalent in Asian countries and in the territory of the former Soviet Union. They are increasingly reported in other areas of the world and are frequently associated with tuberculosis outbreaks and drug resistance. Beijing genotype strains, including W strains, have been characterized by their highly similar multicopy IS6110 restriction fragment length polymorphism (RFLP) patterns, deletion of spacers 1 to 34 in the direct repeat region (Beijing spoligotype), and insertion of IS6110 in the genomic dnaA-dnaN locus. In this study the suitability and comparability of these three genetic markers to identify members of the Beijing lineage were evaluated. In a well-characterized collection of 1,020 M. tuberculosis isolates representative of the IS6110 RFLP genotypes found in The Netherlands, strains of two clades had spoligotypes characteristic of the Beijing lineage. A set of 19 Beijing reference RFLP patterns was selected to retrieve all Beijing strains from the Dutch database. These reference patterns gave a sensitivity of 98.1% and a specificity of 99.7% for identifying Beijing strains (defined by spoligotyping) in an international database of 1,084 strains. The usefulness of the reference patterns was also assessed with large DNA fingerprint databases in two other European countries and for identification strains from the W lineage found in the United States. A standardized definition for the identification of M. tuberculosis strains belonging to the Beijing/W lineage, as described in this work, will facilitate further studies on the spread and characterization of this widespread genotype family of M. tuberculosis strains.