CLONING OF THE CELLULAR RECEPTOR FOR AMPHOTROPIC MURINE RETROVIRUSES REVEALS HOMOLOGY TO THAT FOR GIBBON APE LEUKEMIA-VIRUS
CLONING OF THE CELLULAR RECEPTOR FOR AMPHOTROPIC MURINE RETROVIRUSES REVEALS HOMOLOGY TO THAT FOR GIBBON APE LEUKEMIA-VIRUS
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DOI：
10.1073/pnas.91.1.78
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发表时间：
1994-01-04
影响因子：
11.1
通讯作者：
MILLER, AD
中科院分区：
文献类型：
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作者：
MILLER, DG;EDWARDS, RH;MILLER, AD
逆转录病毒感染的宿主和组织特异性很大程度上取决于介导病毒进入的特定细胞受体。编码这些受体的基因广泛分布在基因组中，迄今为止鉴定的受体没有显示出序列相似性。我们通过筛选引入双嗜性病毒抗性仓鼠细胞的大鼠 cDNA 表达文库，鉴定了双嗜性鼠逆转录病毒 Ram-1 的细胞受体。 656 个氨基酸的受体在两个疏水末端与长臂猿白血病病毒受体同源，但在中央亲水区域高度不同。两种受体似乎都是具有多个跨膜区域的完整膜蛋白。该受体家族的鉴定将有助于定义逆转录病毒及其细胞受体之间的进化关系。
The host and tissue specificity of retrovirus infection is largely determined by specific cellular receptors that mediate virus entry. Genes encoding these receptors are widely distributed in the genome, and the receptors identified to date show no sequence similarity. We have identified the cellular receptor for amphotropic murine retroviruses, Ram-1, by screening a rat cDNA expression library introduced into amphotropic virus-resistant hamster cells. The 656-amino acid receptor is homologous to the gibbon ape leukemia virus receptor at both hydrophobic termini but is highly divergent in the central hydrophilic region. Both receptors appear to be integral membrane proteins having multiple membrane-spanning regions. Identification of this family of receptors will help define the evolutionary relationship between retroviruses and their cellular receptors.