The Mast Cell-restricted Tryptase mMCP-6 Has a Critical Immunoprotective Role in Bacterial Infections*
The Mast Cell-restricted Tryptase mMCP-6 Has a Critical Immunoprotective Role in Bacterial Infections*
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DOI：
10.1074/jbc.m611842200
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发表时间：
2007-07
影响因子：
4.8
通讯作者：
S. Thakurdas;E. Melicoff;L. Sansores-García;Daniel C. Moreira;Youlia M. Petrova;R. Stevens;Roberto Adachi
中科院分区：
文献类型：
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作者：
S. Thakurdas;E. Melicoff;L. Sansores-García;Daniel C. Moreira;Youlia M. Petrova;R. Stevens;Roberto Adachi
尽管已证明肥大细胞缺陷小鼠针对细菌的先天免疫反应减弱，但激活的肥大细胞分泌的最重要的免疫保护因子尚未确定。小鼠肥大细胞蛋白酶 6 是一种四聚体形成类胰蛋白酶。这种丝氨酸蛋白酶在分泌颗粒中含量丰富，并且在细菌攻击时被胞吐。在这里，我们描述了肥大细胞蛋白酶 6 缺失小鼠的产生。我们发现缺乏这种中性蛋白酶的小鼠无法有效地清除腹膜腔中的肺炎克雷伯氏菌，这揭示了这种丝氨酸蛋白酶（可能还有它的人类直系同源物）在先天免疫中的重要作用。
Although it has been shown that mast cell-deficient mice have diminished innate immune responses against bacteria, the most important immunoprotective factors secreted from activated mast cells have not been identified. Mouse mast cell protease 6 is a tetramer-forming tryptase. This serine protease is abundant in the secretory granules and is exocytosed upon bacterial challenge. Here we have described the generation of a mast cell protease-6-null mouse. Our discovery that mice lacking this neutral protease cannot efficiently clear Klebsiella pneumoniae from their peritoneal cavities reveals an essential role for this serine protease, and presumably its human ortholog, in innate immunity.