INCREASED SYSTEMIC HEPARANASE IN RETINAL VEIN OCCLUSION IS ASSOCIATED WITH ACTIVATION OF INFLAMMATION AND THROMBOPHILIA
INCREASED SYSTEMIC HEPARANASE IN RETINAL VEIN OCCLUSION IS ASSOCIATED WITH ACTIVATION OF INFLAMMATION AND THROMBOPHILIA
复制标题
视网膜静脉阻塞时全身乙酰肝素酶升高与炎症和血栓形成倾向的激活有关
DOI：
10.1097/iae.0000000000002374
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发表时间：
2020-02-01
影响因子：
3.3
通讯作者：
Yuan, Ling
中科院分区：
文献类型：
--
作者：
Hu, Yijun;Yu, Yang;Yuan, Ling
目的：探讨视网膜静脉阻塞（RVO）患者全身乙酰肝素酶、炎症标志物和凝血因子活性的水平。研究方法：本前瞻性研究包括18例中央RVO患者，22例分支RVO患者，40例年龄相关性白内障患者作为对照组。血清乙酰肝素酶蛋白水平和活性分别用ELISA和乙酰肝素降解酶测定试剂盒测定。采用ELISA试剂盒检测血清MMP-2、MMP-9、TLR-2和TLR-4水平。用自动分析仪测定凝血因子（V、VII、VIII和IX）的活性。使用Mann-Whitney U检验比较RVO患者和对照受试者之间的上述参数。通过斯皮尔曼相关分析上述两个参数之间的关系。结果如下：与对照组相比，RVO患者的全身乙酰肝素酶蛋白、乙酰肝素酶活性、凝血因子（V、VIII和IX）活性、MMP-2、MMP-9、TLR-2和TLR-4水平较高。全身乙酰肝素酶水平与血清MMP-2、MMP-9、TLR-2、TLR-4水平以及凝血因子VIII和IX活性相关。结论：RVO时全身性乙酰肝素酶升高与全身炎症反应和血液高凝状态有关。
Purpose: To investigate the levels of systemic heparanase, inflammatory markers, and coagulation factor activities in patients with retinal vein occlusion (RVO). Methods: This prospective study included 18 patients with central RVO, 22 patients with branch RVO, and 40 patients with age-related cataract as the control group. Serum heparanase protein levels and activities were measured by ELISA and a heparan degrading enzyme assay kit, respectively. Serum levels of MMP-2, MMP-9, TLR-2, and TLR-4 were measured by ELISA kits. The activities of coagulation factors (V, VII, VIII, and IX) were determined with an autoanalyzer. The Mann-Whitney U test was used to compare the above parameters between patients with RVO and control subjects. The relationship between two of the above parameters was analyzed by Spearman's correlation. Results: Patients with RVO had higher levels of systemic heparanase protein, heparanase activities, coagulation factors' (V, VIII, and IX) activities, MMP-2, MMP-9, TLR-2, and TLR-4 compared with the control group. Systemic heparanase levels were correlated with serum levels of MMP-2, MMP-9, TLR-2, TLR-4, and activities of coagulation factors VIII and IX. Conclusion: Increase of systemic heparanase in RVO is associated with activation of systemic inflammation and blood hypercoagulability.