Heat shock protein 27 expression in the epithelium of periapical lesions
Heat shock protein 27 expression in the epithelium of periapical lesions
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DOI：
10.1097/00004770-200102000-00007
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发表时间：
2001-02-01
影响因子：
4.2
通讯作者：
Travali, S
中科院分区：
文献类型：
--
作者：
Leonardi, R;Villari, L;Travali, S
热休克蛋白（HSP）27是小HSP家族的成员，其在调节上皮细胞生长和分化、伤口愈合、凋亡和细胞保护免受炎性细胞毒性介质中起作用。因此，HSP 27的表达进行了化学研究，在根尖肉芽肿与上皮剩余的马拉色和根尖囊肿。抗HSP 27小鼠单克隆抗体和过氧化物酶标记的链霉亲和素-生物素标准技术被用来研究HSP 27的表达，增殖的上皮细胞休息，和岛屿的上皮和上皮衬里的微囊强烈反应整个所有层，而根尖囊肿上皮衬里主要是一个中等的基底上染色模式。然而，增生的上皮细胞残余物和神经根囊肿都有HSP 27免疫染色强度的过度表达，与局部免疫细胞浸润的存在相一致。HSP 27在根尖周病变中可能发挥多种作用，包括促进上皮细胞的迁移以及增加对坏死和凋亡细胞死亡的抵抗力。
Heat shock protein (HSP) 27 is a member of the small HSP family that plays a part in the regulation of epithelial cell growth and differentiation, wound healing, apoptosis and cell protection against inflammatory cytotoxicity mediators. Thus the expression of HSP27 was investigated immunohistochemically in periapical granulomas with epithelial rests of Malassez and in radicular cysts. Anti-HSP27 mouse monoclonal antibody and peroxidase-labeled streptavidin-biotin standard technique were used to study the expression of HSP27, proliferating epithelial cell rests, and islands of epithelium and epithelial lining of microcysts strongly reacted throughout all layers, whereas radicular cysts epithelial lining presented mainly a moderate suprabasal staining pattern. However both the proliferating epithelial cell rests and the radicular cysts shared an over-expression of HSP27 immunostaining intensity in coincidence with the presence of local infiltration of immune cells. HSP27 may play several roles in periapical lesions that include contributing to the migration of epithelial cell rests and an increased resistance both to necrotic and apoptotic cell deaths.