Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti
Porin Expression Profiles in Haemaphysalis longicornis Infected With Babesia microti
复制标题
DOI：
10.3389/fphys.2020.00502
复制
发表时间：
2020-05
影响因子：
4
通讯作者：
Weiqing Zheng;R. Umemiya-Shirafuji;Qian Zhang;Kiyoshi Okado;P. F. Adjou Moumouni;Hiroshi Suzuki;Haiying Chen;Mingming Liu;X. Xuan
中科院分区：
文献类型：
--
作者：
Weiqing Zheng;R. Umemiya-Shirafuji;Qian Zhang;Kiyoshi Okado;P. F. Adjou Moumouni;Hiroshi Suzuki;Haiying Chen;Mingming Liu;X. Xuan
孔蛋白基因广泛分布于各种生物中，在调节吸血节肢动物的病原体感染中具有关键作用。然而，迄今为止，关于长角血蜱（人和动物疾病的重要媒介）的孔蛋白基因的信息仍然未知。在本研究中，我们从H.并在感染和未感染小巴虫的H. longicornis中检测其表达水平。longicornis蜱在发育阶段。我们还分析了孔蛋白与蜱吸血和巴贝虫感染的相关功能，以及孔蛋白与孔蛋白相关凋亡基因如B细胞淋巴瘤（Bcl）、细胞色素复合物（Cytc）、半胱天冬酶2（Cas 2）和半胱天冬酶8（Cas 8）之间的关系。H.长角牛孔蛋白cDNA全长849 bp，编码282个氨基酸。结构域分析表明，该蛋白含有6个决定因素的电压门控和两个多肽结合位点。孔蛋白mRNA水平在1日龄和7日龄蛋之间没有显著差异。在若虫阶段，未进食、12 h部分进食（12 HPF）、1 d部分进食（1 dPF）、2 dPF饱食后0 d进食（0 dAE）和饱食后0 d进食（0 dAE）的孔蛋白表达水平高于2 dAE时的孔蛋白表达水平。与2dAE时相比，2dPF组的Cytc和Cas 2 mRNA水平更高。除了在1 dPF和0-1 dAE，在血液喂养期间，感染的猪与未感染的猪相比，孔蛋白表达水平似乎更高。尤其是最高的B。田鼠负担负影响孔蛋白mRNA水平在两个成年人和女性。孔蛋白基因敲除可影响小鼠体重和巴氏杆菌感染水平，并显著下调Cytc和Bcl的表达水平。长角雌蜱此外，本研究还表明，B. microti Gray品系的若虫和雌虫。从吸血到充血后，长角龙在充血时或充血前后达到高峰。综上所述，本研究中进行的研究表明H。长角孔蛋白可能干扰血液摄食和B.真菌感染
The porin gene is widely disseminated in various organisms and has a pivotal role in the regulation of pathogen infection in blood-sucking arthropods. However, to date, information on the porin gene from the Haemaphysalis longicornis tick, an important vector of human and animal diseases, remains unknown. In this study, we identified the porin gene from H. longicornis and evaluated its expression levels in Babesia microti-infected and -uninfected H. longicornis ticks at developmental stages. We also analyzed porin functions in relation to both tick blood feeding and Babesia infection and the relationship between porin and porin-related apoptosis genes such as B-cell lymphoma (Bcl), cytochrome complex (Cytc), caspase 2 (Cas2), and caspase 8 (Cas8). The coding nucleotide sequence of H. longicornis porin cDNA was found to be 849 bp in length and encoded 282 amino acids. Domain analysis showed the protein to contain six determinants of voltage gating and two polypeptide binding sites. Porin mRNA levels were not significantly different between 1-day-laid and 7-day-laid eggs. In the nymphal stage, higher porin expression levels were found in unfed, 12-h-partially-fed (12 hPF), 1-day-partially-fed (1 dPF), 2 dPF nymphs and nymphs at 0 day post-engorgement (0 dAE) vs. nymphs at 2 dAE. Cytc and Cas2 mRNA levels were higher in 2 dPF nymphs in contrast to nymphs at 2 dAE. Porin expression levels appeared to be higher in the infected vs. uninfected nymphs during blood feeding except at 1 dPF and 0–1 dAE. Especially, the highest B. microti burden negatively affected porin mRNA levels in both nymphs and female adults. Porin knockdown affected body weight and Babesia infection levels and significantly downregulated the expression levels of Cytc and Bcl in H. longicornis female ticks. In addition, this study showed that infection levels of the B. microti Gray strain in nymphal and female H. longicornis peaked at or around engorgement from blood feeding to post engorgement. Taken together, the research conducted in this study suggests that H. longicornis porin might interfere with blood feeding and B. microti infection.