Action of the bacterial neutral protease, dispase, on cultured cells and its application to fluid suspension culture with a review on biomedical application of this protease.
Action of the bacterial neutral protease, dispase, on cultured cells and its application to fluid suspension culture with a review on biomedical application of this protease.
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细菌中性蛋白酶、分散酶对培养细胞的作用及其在液体悬浮培养中的应用，并对该蛋白酶的生物医学应用进行了综述。
DOI：
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发表时间：
1986
期刊：
影响因子：
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通讯作者：
T. Matsumura
中科院分区：
文献类型：
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作者：
M. Nagata;T. Matsumura
利用21个哺乳动物细胞系对多粘芽孢杆菌中性蛋白酶的细胞分散能力、细胞毒性和对细胞生长的影响进行了研究。先前的观察结果表明，成纤维细胞样细胞被这种蛋白酶从培养底物中分离出来，并解离成单分散的细胞悬浮液，而上皮样细胞被分离但未完全解离，这一点得到了普遍证实，尽管注意到这些一般规则的例外情况。在此，我们利用疫病开发了悬浮培养和单细胞镀技术，分别描述了6株悬浮培养和3株单细胞镀的成功条件。这些技术的关键因素是在生长培养基中选择低浓度的蛋白酶以避免其细胞毒性作用，并在培养基中选择适当的血清浓度以使蛋白酶保持活性。综述了近年来疾病在生物医学中的应用。
The bacterial neutral protease from Bacillus polymyxa, dispase, has been characterized using 21 mammalian cell lines in its cell-dispersing capability, cytotoxicity, and effects on cell growth. The previous observation that fibroblast-like cells are detached by this protease from culture substrate, as well as dissociated into a monodisperse cell suspension, while that epithelial-like cells are detached but not completely dissociated, was generally confirmed, although exceptions to these general rules were noted. Efforts were made here by the use of dispase to develop techniques for suspension culture and single cell plating: Successful conditions of suspension culture for 6 lines, and single cell plating for 3 lines were respectively described. Key factors in these techniques were choice of a low concentration of dispase in growth media to avoid its cytotoxic effect, and choice of appropriate serum concentration in the medium to allow this protease to remain active. Recent literatures on the application of dispase in biomedical science were reviewed.