Microscopic Analysis of the Chlamydia abortus Inclusion and Its Interaction with Those Formed by Other Chlamydial Species.
Microscopic Analysis of the Chlamydia abortus Inclusion and Its Interaction with Those Formed by Other Chlamydial Species.
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流产衣原体包涵体及其与其他衣原体物种形成的包涵体的相互作用的显微分析。
DOI：
10.1128/iai.00499-21
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发表时间：
2022
影响因子：
3.1
通讯作者：
Rockey,DanielD
中科院分区：
文献类型：
--
作者：
Garvin,LotishaE;DeBoer,AddisonG;Carrell,StevenJ;Wang,Xisheng;Rockey,DanielD
衣原体是专有的细胞内病原体，在生长过程中在一个特征不佳的寄生性液泡(包涵体)内发展和繁殖。流产衣原体是绵羊和其他反刍动物的主要病原体，其包涵体发育特点较差。我们使用免疫荧光显微镜、定量培养和定量聚合酶链式反应来检测流产线虫包涵体的发育，并研究流产线虫包涵体与其他物种形成的包涵体之间的相互作用。这些研究中使用的抗体包括生产设施中自然感染流产线虫的母羊的血清。经常发现多种夹杂物。被流产感染的细胞，即使在感染人群中感染的复数很低。用感染绵羊的血清标记固定细胞，发现纤维结构从包涵体延伸到宿主细胞的细胞质中。在混合感染细胞中，流产弧菌包涵体与鼠疫杆菌包涵体和鹦鹉热弧菌包涵体融合。流产衣原体和豚鼠衣原体形成的包裹体与沙眼衣原体、肺炎衣原体和胸衣原体形成的包裹体不融合。包涵体的融合能力与物种间的总体基因组亲缘关系以及包涵体膜蛋白INCA中的序列相似性相关。定量聚合酶链式反应结果表明，c.在与豚鼠共感染期间，流产的生长速度较慢，而豚鼠的生长不受影响。收集的数据增加了我们对这种重要的人畜共患病病原体包涵体发展的理解。
The Chlamydiae are obligate intracellular pathogens that develop and multiply within a poorly characterized parasitophorous vacuole (the inclusion) during growth. Chlamydia abortus is a major pathogen of sheep and other ruminants, and its inclusion development is poorly characterized. We used immunofluorescence microscopy, quantitative culture, and qPCR to examine C. abortus inclusion development and to examine the interaction of C. abortus inclusions with those formed by other species. Antibodies used in these studies include sera from ewes from production facilities that were naturally infected with C. abortus. Multiple inclusions are often found inC. abortus-infected cells, even in populations infected at very low multiplicity of infection. Labeling of fixed cells with sera from infected sheep revealed fibrous structures that extend away from the inclusion into the cytoplasm of the host cell. C. abortus inclusions fused with C. caviae and C. psittaci inclusions in coinfected cells. Inclusions formed by C. abortus and C. caviae did not fuse with inclusions formed by C. trachomatis, C. pneumoniae, or C. pecorum. The ability of inclusions to fuse was correlated with the overall genomic relatedness between species, and with sequence similarity in the inclusion membrane protein IncA. Quantitative PCR data demonstrated thatC. abortusgrows at a decreased rate during coinfections with C. caviae, while C. caviae growth was unaffected. The collected data add depth to our understanding of inclusion development in this significant zoonotic veterinary pathogen.