IDENTIFICATION AND SEQUENCE OF A NA+-LINKED GENE FROM THE MARINE BACTERIUM ALTEROMONAS-HALOPLANKTIS WHICH FUNCTIONALLY COMPLEMENTS THE DAGA GENE OF ESCHERICHIA-COLI
IDENTIFICATION AND SEQUENCE OF A NA+-LINKED GENE FROM THE MARINE BACTERIUM ALTEROMONAS-HALOPLANKTIS WHICH FUNCTIONALLY COMPLEMENTS THE DAGA GENE OF ESCHERICHIA-COLI
复制标题
DOI：
10.1111/j.1365-2958.1992.tb01444.x
复制
发表时间：
1992-09-01
影响因子：
3.6
通讯作者：
MACLEOD, RA
中科院分区：
文献类型：
--
作者：
MACLEOD, PR;MACLEOD, RA
从海洋细菌Alteromonas haloacetatis ATCC 19855的质粒基因组DNA文库中发现了一个4.0 kb的片段，在Na+存在下与大肠杆菌的dagA基因互补。我们已经完全测序这个片段和Na+连接的D-丙氨酸甘氨酸通透酶基因（dagA）的片段上的位置已被确定的互补。预测的载体蛋白由542个氨基酸残基组成（M（r）58955）。其亲水性特征表明它由八个跨膜片段组成，在片段6和7之间具有长的亲水区域。该酶与大肠杆菌的Na ~+/脯氨酸通透酶有很大的相似性。大肠杆菌和鼠伤寒沙门氏菌以及人和兔肠道Na+/葡萄糖共转运蛋白。
A 4.0 kb fragment from a plasmid genomic DNA library of the marine bacterium Alteromonas haloplanktis ATCC 19855 was found in the presence of Na+ to complement the dagA gene of Escherichia coli. We have completely sequenced this fragment and the position of the Na+-linked D-alanine glycine permease gene (dagA) on the fragment has been determined by complementation. The predicted carrier protein consists of 542 amino acid residues (M(r) 58955). Its hydropathy profile suggests it is composed of eight transmembrane segments with a long hydrophilic region between segments six and seven. Significant similarity has been found between this Na+-linked permease and the Na+/proline permeases of E. coli and Salmonella typhimurium and the human and rabbit intestinal Na+/glucose cotransporters.