Cytokine responses of oral epithelial cells to Porphyromonas gingivalis infection
Cytokine responses of oral epithelial cells to Porphyromonas gingivalis infection
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DOI：
10.1177/00220345000790101301
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发表时间：
2000-10-01
影响因子：
7.6
通讯作者：
Papapanou, PN
中科院分区：
文献类型：
--
作者：
Sandros, J;Karlsson, C;Papapanou, PN
越来越多的证据表明，上皮细胞不仅是机械屏障，而且是先天免疫系统的重要组成部分。本研究旨在探讨牙龈卟啉单胞菌感染后口腔上皮细胞的细胞因子反应。用牙龈卟啉单胞菌感染KB细胞系和牙周袋上皮的原代培养物，通过抗生素保护试验评估细菌侵袭，并通过原位杂交和免疫组织化学检查白细胞介素-1 β、白细胞介素-6、白细胞介素-8和肿瘤坏死因子-α的表达。我们观察到牙龈卟啉单胞菌诱导强烈的细胞因子应答，在KB细胞和原代培养物中与感染菌株的粘附/侵袭潜力呈正相关。这些发现表明，牙周袋的上皮细胞是免疫系统的一个组成部分，引发细胞因子对细菌的挑战。在这种情况下，牙龈卟啉单胞菌的粘附/侵袭表型似乎有助于致病性。
Accumulating evidence indicates that epithelia are not merely mechanical barriers but also important elements of the innate immune system. The present study was performed to examine cytokine responses of oral epithelial cells after infection with the periodontal pathogen Porphyromonas gingivalis. The KB-cell line and primary cultures of periodontal pocket epithelium were infected with P. gingivalis for assessment of bacterial invasion by an antibiotic protection assay, and examinination of expression of interleukin-l beta, interleukin-6, interleukin-8, and tumor necrosis factor-alpha by in situ hybridization and immunohistochemistry. We observed that P. gingivalis induces a strong cytokine response, positively correlated with the adhesive/invasive potential of the infecting strain, in both KB cells and primary cultures. These findings indicate that the epithelial cells of the periodontal pocket are an integral part of the immune system, eliciting cytokine responses to a bacterial challenge. In this context, the adhesive/invasive phenotype of P. gingivalis appears to contribute to pathogenicity.