Early-Life Exposure to the Chinese Famine Is Associated with Higher Methylation Level in the INSR Gene in Later Adulthood
Early-Life Exposure to the Chinese Famine Is Associated with Higher Methylation Level in the INSR Gene in Later Adulthood
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DOI：
10.1038/s41598-019-38596-6
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发表时间：
2019-03
影响因子：
4.6
通讯作者：
Zhenghe Wang;Jie-Yun Song;Yanhui Li;B. Dong;Z. Zou;Jun Ma
中科院分区：
文献类型：
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作者：
Zhenghe Wang;Jie-Yun Song;Yanhui Li;B. Dong;Z. Zou;Jun Ma
我们对来自中国两个省份的 235 名受试者进行了研究，研究了早期中国饥荒暴露与与生长和代谢相关的 INSR（hg18，chr19：7110130-7110574）和 CPT1A（hg18，chr11：68286513-68286952）DNA 甲基化之间的关联。根据出生日期将受试者分为产前饥荒暴露组和非暴露组。使用 Sequenom 的 MassARRAY 系统检测外周白细胞中 INSR 基因位点的 DNA 甲基化。采用二独立样本检验比较暴露组与非暴露组之间的差异。胎儿时期暴露于中国饥荒的个体INSR DNA甲基化水平高于未暴露组(d= 3.3%,P= 0.006)。 INSR 观察到饥荒暴露与省份之间存在显着的交互作用（Pinteraction< 0.001）。 INSR DNA甲基化水平与甘油三酯呈正相关（β= 0.011，P= 0.021），与高密度脂蛋白胆固醇呈负相关（β= −0.039，P= 0.021）。此外，在重度暴露地区，暴露组的肉类消耗量高于非暴露组。产前经历的中国饥荒加上晚年的饮食习惯可能会调节表观基因组。
We examined the association between the China famine exposure in early life and DNA methylation ofINSR(hg18, chr19:7110130-7110574) andCPT1A(hg18, chr11: 68286513-68286952) related to growth and metabolism in 235 subjects selected from two provinces in China. The subjects were categorized into prenatal famine-exposed group and non-exposed group based on their birthdates. DNA methylation at theINSRgene locus was assayed from peripheral white blood cells using the Sequenom’s MassARRAY system. Two dependent samplest-test was used to compare the difference between the exposed group and non-exposed group. DNA methylation level ofINSRwas higher among individuals who exposed to the China famine in the fetus than that of non-exposed group (d= 3.3%,P= 0.006). A significant interaction between famine exposure and province was observed forINSR(Pinteraction< 0.001). DNA methylation level ofINSRwas positively associated with triglyceride (β= 0.011,P= 0.021), and negatively associated with high-density lipoprotein cholesterol (β= −0.039,P= 0.021). Moreover, exposed group had higher meat consumption than non-exposed group in severe exposure area. Prenatal exposure to the China famine plus later life eating habits might regulate epigenome.