Autocrine growth and progression of murine X-ray-induced T cell lymphomas.
Autocrine growth and progression of murine X-ray-induced T cell lymphomas.
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小鼠 X 射线诱导的 T 细胞淋巴瘤的自分泌生长和进展。
DOI：
10.1002/j.1460-2075.1986.tb04426.x
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
Vogt,M
中科院分区：
文献类型：
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作者：
Haas,M;Mally,MI;Bogenberger,JM;Bogart,MH;Buchanan,MA;Augery-Bourget,Y;Hyman,R;Vogt,M
x射线诱导的小鼠T细胞淋巴瘤的原发肿瘤包括自分泌、生长因子依赖的细胞。我们已经培养了原发性x射线诱导的胸腺淋巴瘤细胞系（PXTLs），在最小化细胞从因子依赖到因子独立的进展的条件下。所有生长的PXTL细胞系都分泌一种支持自身生长的生长因子，我们称之为淋巴瘤生长因子LGF。lgf依赖性细胞无致瘤性或致瘤性较差，不能在软琼脂中克隆，在c-myc或Pim-1区域没有可检测到的重排，具有接近二倍体或假二倍体的核型，没有15号或17号染色体三体的证据。pxtl分泌的LGF没有白细胞介素1、2或3活性，也不分泌LGF的细胞合成可检测的IL-1、2或-3 mRNA。在特定的生物测定中，LGF不含可检测到的干扰素或GM-CSF活性。纯化的EGF、TGF β和白细胞介素制剂对依赖于TGF的PXTL细胞无活性。因此，LGF似乎是一种新的生长因子，是非进展性T淋巴瘤细胞增殖所必需的。在进展过程中，PXTL细胞变得不依赖于生长因子，在体内具有高度的致瘤性，在软琼脂中克隆，并具有含有许多染色体畸变的近三倍体核型。因此，在x射线诱导的淋巴瘤发生中，自分泌、lgf依赖期先于进展期，其特征是myc和/或Pim-1区域的重排以及核型中可见的许多染色体畸变。图片
Primary tumors of X-ray-induced murine T cell lymphomas comprise autocrine, growth factor-dependent cells. We have grown cell lines from primary X-ray-induced thymic lymphomas (PXTLs) under conditions which minimize the progression of the cells from factor dependence to factor independence. All (22) PXTL lines grown secrete a growth factor which supports their own growth and which we will call lymphoma growth factor LGF. LGF-dependent cells are non-tumorigenic or poorly tumorigenic, do not clone in soft agar, have no detectable rearrangements in the c-myc or Pim-1 region and possess near diploid or pseudodiploid karyotypes without evidence for trisomy of chromosomes nos. 15 or 17. PXTL-secreted LGF has no interleukin 1, 2, or 3 activity nor do LGF-secreting cells synthesize detectable IL-1, -2, or -3 mRNA. LGF contains no detectable interferon or GM-CSF activity in specific bioassays. Purified EGF, TGF beta, and interleukin preparations are inactive on LGF-dependent PXTL cells. Thus LGF appears to be a new growth factor that is required for the proliferation of non-progressed T lymphoma cells. Upon progression PXTL cells become growth factor independent, are highly tumorigenic in vivo, clone in soft agar, and assume a near triploid karyotype containing numerous chromosomal aberrations. Thus in X-ray-induced lymphomagenesis an autocrine, LGF-dependent phase precedes the progressed phase characterized by rearrangements in the myc and/or Pim-1 regions as well as by many chromosomal aberrations visible in the karyotype. Images