Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats
Electrical high frequency stimulation of the caudate nucleus induces local GABA outflow in freely moving rats
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DOI：
10.1016/j.jneumeth.2006.07.023
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发表时间：
2007-01-30
影响因子：
3
通讯作者：
Moser, A.
中科院分区：
文献类型：
--
作者：
Hiller, A.;Loeffler, S.;Moser, A.
高频电刺激苍白球内侧核或丘脑底核对晚期帕金森病有有益的运动效应。然而，这些临床结果背后的机制仍不清楚。从以前的体外研究，它提出了γ-氨基丁酸（GABA）系统参与的电高频刺激（HFS）的有效性。在这些实验中，我们开发了一种在体内模型，允许同时和并列微透析和HFS的电脉冲的124 Hz的自由移动大鼠的尾状核。GABA和谷氨酸流出物通过微透析技术进行采样，并在柱前邻苯二甲醛亚硫酸盐衍生化后使用HPLC电化学检测进行定量。作为最突出的结果，我们可以证明，高频刺激显着增加基础GABA流出，而不影响谷氨酸水平在自由活动的大鼠。(c)2006 Elsevier B. V.保留所有权利。
Electrical high frequency stimulation of the globus pallidus internus or the subthalamic nucleus has beneficial motor effects in advanced Parkinson's disease. The mechanisms underlying these clinical results remain, however, unclear. From previous in vitro studies it is proposed that the gamma-aminobutyric acid (GABA) system is involved in the effectiveness of electrical high frequency stimulation (HFS). In these experiments, we developed an in vivo model that allows for simultaneous and collocated microdialysis and HFS by electrical pulses of 124 Hz in the caudate nucleus of freely moving rats. GABA and glutamate outflow were sampled by microdialysis technique and quantified after pre-column o-phthaldialdehyde sulphite derivatization using HPLC with electrochemical detection. As the most outstanding result, we could demonstrate that high frequency stimulation significantly increased basal GABA outflow without affecting glutamate levels in freely moving rats. (c) 2006 Elsevier B.V. All rights reserved.