Methanofervidicoccus abyssi gen. nov., sp. nov., a hydrogenotrophic methanogen, isolated from a hydrothermal vent chimney in the Mid-Cayman Spreading Center, the Caribbean Sea
Methanofervidicoccus abyssi gen. nov., sp. nov., a hydrogenotrophic methanogen, isolated from a hydrothermal vent chimney in the Mid-Cayman Spreading Center, the Caribbean Sea
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阿比产甲烷菌 (Methanofervidicbacter abyssi)
DOI：
10.1099/ijsem.0.003297
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发表时间：
2019
影响因子：
2.8
通讯作者：
Takai Ken
中科院分区：
文献类型：
--
作者：
Sakai Sanae;Takaki Yoshihiro;Miyazaki Masayuki;Ogawara Miyuki;Yanagawa Katsunori;Miyazaki Junichi;Takai Ken
从加勒比海中开曼扩张中心毕贝喷口采集的深海热液喷口烟囱样品中分离到一株新的亲水产甲烷菌HHBT。细胞不活动，呈规则至不规则的球状，有多个鞭毛。新菌株生长在60~80 °C、pH 5.0~7.4、含1~4 %(w/v)氯化钠的条件下。该菌株利用H2/CO2作为生长和产甲烷的唯一底物。16S rRNA和mcrA基因序列的系统发育分析和比较基因组分析的结果表明，HHBT是甲烷球菌目的一个成员，与甲烷热球菌属和甲氧基托里斯属的成员关系密切。在16SRRNA基因序列比较中，亲缘关系最近的是甲氧西林甲烷杆菌IH1T和黑甲虫Kol 5，同源性分别为93 %和Mc-S-70。在推导的氨基酸序列相似性方面，甲环虫Mc-S-70的同源性为92 %。HHBT与甲烷热球菌属和甲氧菌属的ANI值为69~72 % ，AAI值为66~70 %。虽然HHBT的许多形态和生理特征与甲烷热球菌属和甲氧菌属的相似，但它们对抗生素的敏感性、甲酸盐的利用、生长温度和盐范围的差异是可以区分的。在这些表型、系统发育和基因组特征的基础上，我们认为HHBT菌株是一个新种，属于一个新属--深水甲烷假单胞菌。Nov.，sp.11月模式菌株为HHBT(=JCM 32161T=DSM 105918T)。
A novel hydrogenotrophic methanogen, strain HHBT, was isolated from a deep-sea hydrothermal vent chimney sample collected from Beebe Vent Field at the Mid-Cayman Spreading Center, Caribbean Sea. The cells were non-motile regular to irregular cocci possessing several flagella. The novel isolate grew at 60–80 °C, pH 5.0–7.4 and with 1–4 % of NaCl (w/v). The isolate utilized H2/CO2as the only substrates for growth and methane production. The results of phylogenetic analyses of both 16S rRNA andmcrAgene sequences and comparative genome analysis indicated that HHBTrepresented a member of the orderMethanococcales, and was closely related to the members of the generaMethanothermococcusandMethanotorris. The most closely related species wereMethanothermococcus okinawensisIH1TandMethanotorris igneusKol 5Tin comparison of 16S rRNA gene sequences (each with 93 % identity), andMethanotorris formicicusMc-S-70Tin the case of deduced amino acid sequence similarity ofmcrAgenes (92 % similarity). The ANI and AAI values between HHBTand the members of the generaMethanothermococcusandMethanotorriswere 69–72 % and 66–70 %, respectively. Although many of the morphological and physiological characteristics were quite similar between HHBTand the species of the generaMethanothermococcusandMethanotorris, they were distinguishable by the differences in susceptibility to antibiotics, formate utilization, growth temperature and NaCl ranges. On the basis of these phenotypic, phylogenetic and genomic properties, we propose that strain HHBTrepresents a novel species, of a novel genus,Methanofervidicoccus abyssigen. nov., sp. nov. The type strain is HHBT(=JCM 32161T=DSM 105918T).