An Action Spectrum of the riboflavin-photosensitized inactivation of lambda phage
An Action Spectrum of the riboflavin-photosensitized inactivation of lambda phage
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DOI：
10.1562/2004-08-25-ra-292.1
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发表时间：
2005-03-01
影响因子：
3.3
通讯作者：
Platz, M
中科院分区：
文献类型：
--
作者：
Martin, CB;Wilfong, E;Platz, M
在266~575 nm处测定了核黄素(Rb)致敏灭活噬菌体的作用光谱。在304 nm以下，RB通过屏蔽原本会直接吸收的辐射来抑制噬菌体的还原。在308-525 nm范围内，Rb对噬菌体的灭活有敏化作用。由于Rb与噬菌体的结合以及噬菌体结合黄素的吸收曲线相对于游离黄素在磷酸盐缓冲溶液中的移动，在320 nm和500 nm处观察到了增强的噬菌体还原。320和500 nm处的增强灭活和360~410 nm处的抑制灭活也受内滤膜效应的影响。
The Action Spectrum of riboflavin (RB) sensitized inactivation of lambda phage was determined between 266 and 575 nm. Below 304 nm, RB depresses the phage reduction by screening phage from radiation that it would otherwise absorb directly. Between 308 and 525 nm, RB sensitizes the inactivation of phage. Enhanced phage reduction is observed at 320 and 500 nm because of binding of RB to the phage and the shifting of the absorption curve of the phage-bound flavin relative to free flavin in phosphate-buffered saline. Enhanced inactivation at 320 and 500 nm and depressed phage inactivation between 360 and 410 nm is also influenced by the inner filter effect.