Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes

Heterologous expression of the Pleurotus ostreatus MnP3 gene by the laccase gene promoter in Lentinula edodes
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漆酶基因启动子在香菇中异源表达平菇MnP3基因

DOI：
10.1080/09168451.2017.1332977
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发表时间：
2017
期刊：
Bioscience, Biotechnology, and Biochemistry
影响因子：
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通讯作者：
Yoshino Fumihiko
Yoshino Fumihiko
中科院分区：
--
文献类型：
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作者：
Sato Toshitsugu;Irie Toshikazu;Yoshino Fumihiko

文献摘要

相似文献

香菇（Lentinula edodes）是亚洲最重要的食用蘑菇之一，具有强大的木质素分解系统。本研究从平菇（pleurotus ostreatusl）中引入了由l驱动的锰过氧化物酶（MnP, EC 1.11.1.13）基因。edodeslacase 1基因启动子。edodesfor表达式。结果表明，该菌株表达了重组基因，且MnP活性高于野生型菌株。
Lentinula edodes(shiitake), which have a powerful ligninolytic system, is one of the most important edible mushrooms in Asia. In this study, we introduced the manganese peroxidase (MnP, EC 1.11.1.13) gene fromPleurotus ostreatusdriven byL. edodeslaccase 1 gene promoter intoL. edodesfor expression. The resulting transformant expressed the recombinant gene and showed a higher level of MnP activity than that of the wild-type strain.