Influence of medium components and fermentation conditions on the production of bacteriocin(s) by Bacillus licheniformis AnBa9
Influence of medium components and fermentation conditions on the production of bacteriocin(s) by Bacillus licheniformis AnBa9
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DOI：
10.1016/j.biortech.2008.07.027
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发表时间：
2009-01-01
影响因子：
11.4
通讯作者：
Gunasekaran, Paramasamy
中科院分区：
文献类型：
--
作者：
Anthony, Thangamani;Rajesh, Thangamani;Gunasekaran, Paramasamy
近年来，抗菌肽作为一种替代治疗药物和食品等产品的腐败和变质，越来越受到人们的关注。从屠宰废水底泥中分离抗菌肽产生菌。其中一株地衣芽孢杆菌对几种革兰氏阳性菌的生长有抑制作用。采用响应面中心组合旋转设计法对抗菌肽发酵培养基和发酵条件进行优化。乳糖，NH4NO3，酵母提取物和NaCl和环境因素，如pH值，温度和孵育时间被选为变量。其中，高浓度的酵母抽提物和NaCl分别对抗菌肽的产量和比活力有促进作用。碱性pH和高温有利于B产生抗菌肽。地衣AnBa 9.在优化条件下，B.地衣AnBa 9的抗菌肽产量比未优化的条件高25倍。对B抗菌肽的生化特性进行了研究。地衣AnBa 9菌株鉴定为细菌素型。(C)2008爱思唯尔有限公司保留所有权利。
Recently, antibacterial peptides are gaining more attention as an alternative therapeutics and food and other products from spoilage and deterioration. Antibacterial peptide producing strains were isolated from sediments of slaughterhouse sewage wastes. One among them, identified as Bacillus licheniformis inhibited the growth of several gram positive bacteria. Response surface methodology with central composite rotary design was used for optimization of fermentation medium and conditions for antibacterial peptide production. Lactose, NH4NO3, yeast extract and NaCl and environmental factors such as pH, temperature and incubation period were selected as variables. Among ingredients, high concentration of yeast extract and NaCl had a positive effect on antibacterial peptide production and specific activity, respectively. Alkaline pH and high temperature favoured the production of antibacterial peptide by B. licheniformis AnBa9. Under optimized condition, B. licheniformis AnBa9 produced 25-fold higher production of antibacterial peptide than the un-optimized condition. Biochemical characteristics of the antibacterial peptides of B. licheniformis AnBa9 revealed that they are of bacteriocin type. (C) 2008 Elsevier Ltd. All rights reserved.