Lsh, a member of the SNF2 family, is required for genome-wide methylation
Lsh, a member of the SNF2 family, is required for genome-wide methylation
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DOI：
10.1101/gad.929101
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发表时间：
2001-11-15
影响因子：
10.5
通讯作者：
Muegge, K
中科院分区：
文献类型：
--
作者：
Dennis, K;Fan, T;Muegge, K
哺乳动物基因组的甲基化模式被认为对发育至关重要。指定特定基因组位点进行甲基化的精确机制尚不清楚。Lsh的靶向缺失导致围产期死亡，发育相当正常。然而，我们在这里报告说，Lsh（-/-）小鼠在整个基因组中表现出大量的甲基化丢失。低甲基化基因座包括重复元件和单拷贝基因。这表明整个基因组甲基化并不是正常胚胎发生所必需的。基于Lsh与其他SNF 2染色质重塑蛋白的相似性，这表明染色质的改变影响小鼠的整体甲基化模式。
Methylation patterns of the mammalian genome are thought to be crucial for development. The precise mechanisms designating specific genomic loci for methylation are not known. Targeted deletion of Lsh results in perinatal lethality with a rather normal development. We report here, however, that Lsh(-/-) mice show substantial loss of methylation throughout the genome. The hypomethylated loci comprise repetitive elements and single copy genes. This suggests that global genomic methylation is not absolutely required for normal embryogenesis. Based on the similarity of Lsh to other SNF2 chromatin remodeling proteins, it suggests that alteration of chromatin affects global methylation patterns in mice.