The nanoscale organization of the B lymphocyte membrane
The nanoscale organization of the B lymphocyte membrane
复制标题
DOI：
10.1016/j.bbamcr.2014.11.010
复制
发表时间：
2015-04-01
影响因子：
5.1
通讯作者：
Reth, Michael
中科院分区：
文献类型：
--
作者：
Maity, Palash Chandra;Yang, Jianying;Reth, Michael
Singer和Nicolson的流体镶嵌模型正确地预测了质膜（PM）形成含有许多完整的跨膜蛋白的脂质双层。该模型还表明，这些蛋白质中的大多数是随机分散和自由扩散的部分。最初，这种动态的、相当无组织的膜的观点得到了早期使用光学显微镜对细胞表面的观察的支持。然而，最近对低于可见光衍射极限（类似于250 nm）的PM的研究表明，在纳米尺度下，膜是高度组织化和区室化的结构。淋巴细胞对于研究这种纳米级膜组织特别有用，因为它们作为单细胞生长，并且不会永久参与可能影响膜组织的组织内的细胞：细胞接触。在这篇综述中，我们描述了可以用来更好地研究蛋白质：蛋白质相互作用和淋巴细胞膜蛋白的纳米组织的方法，重点是B细胞抗原受体（BCR）。此外，我们讨论了可能产生和维持这些膜结构的因素。这篇文章是一个特殊问题的一部分，题为：纳米膜组织和信号。2014年，作者。由Elsevier B. V.发布这是CC BY-NC-ND许可下的开放获取文章
The fluid mosaic model of Singer and Nicolson correctly predicted that the plasma membrane (PM) forms a lipid bilayer containing many integral trans-membrane proteins. This model also suggested that most of these proteins were randomly dispersed and freely diffusing moieties. Initially, this view of a dynamic and rather unorganized membrane was supported by early observations of the cell surfaces using the light microscope. However, recent studies on the PM below the diffraction limit of visible light (similar to 250 nm) revealed that, at nanoscale dimensions, membranes are highly organized and compartmentalized structures. Lymphocytes are particularly useful to study this nanoscale membrane organization because they grow as single cells and are not permanently engaged in cell:cell contacts within a tissue that can influence membrane organization. In this review, we describe the methods that can be used to better study the protein:protein interaction and nanoscale organization of lymphocyte membrane proteins, with a focus on the B cell antigen receptor (BCR). Furthermore, we discuss the factors that may generate and maintain these membrane structures. This article is part of a Special Issue entitled: Nanoscale membrane organisation and signalling. 0 2014 The Authors. Published by Elsevier B.V. This is an open access article under the CC BY-NC-ND license