Accurate and objective preoperative diagnosis of thyroid papillary carcinomas by reverse transcription-PCR detection of oncofetal fibronectin messenger RNA in fine-needle aspiration biopsies.
Accurate and objective preoperative diagnosis of thyroid papillary carcinomas by reverse transcription-PCR detection of oncofetal fibronectin messenger RNA in fine-needle aspiration biopsies.
复制标题
通过细针抽吸活检中癌胎纤连蛋白信使 RNA 的逆转录 PCR 检测，准确、客观地术前诊断甲状腺乳头状癌。
作者：
Toru Takano;Akira Miyauchi;T. Yokozawa;F. Matsuzuka;Gang Liu;T. Higashiyama;Shinji Morita;Kanji Kuma;Nobuyuki Amino
最近，据报道，癌胎纤连蛋白 mRNA 在甲状腺乳头状癌和未分化癌中表达受限。在这项研究中，通过从用于细针抽吸活检的针内剩余细胞中提取RNA，我们建立了一种对这些癌症进行基因诊断的新方法，且不会进一步侵入患者（抽吸活检-逆转录-PCR，ABRP）。从 177 例可疑恶性的甲状腺结节细针抽吸活检中提取 RNA，然后将通过反转录 PCR 检测癌胎纤连蛋白 mRNA 做出的基因诊断与细胞学诊断进行比较。通过细胞学检查诊断为乳头状癌或间变性癌的 37 份样本中，有 35 份（94.6%）的基因诊断结果呈阳性，而在细胞学诊断为两种癌阴性的 109 份样本中，只有 4 份（3.7%）显示阳性结果。在所有病例中，50例患者接受了手术，并做出了组织学诊断。该方法的敏感性和特异性分别为96.9%和100%。使用遗传和细胞学方法的联合检查可能有助于乳头状癌和未分化癌的更精确的术前诊断。
Recently, the restricted expression of oncofetal fibronectin mRNA was reported in thyroid papillary and anaplastic carcinomas. In this study, by extracting RNA from the leftover cells inside the needles used for fine-needle aspiration biopsy, we establish a new method for gene diagnosis of these carcinomas without further invasiveness to the patient (aspiration biopsy-reverse transcription-PCR, ABRP). RNA was extracted from 177 fine-needle aspiration biopsies of thyroid nodules that were suspicious for malignancy, and then the gene diagnoses made by reverse transciption-PCR detection of oncofetal fibronectin mRNA were compared with cytological diagnoses. Thirty-five (94.6%) of 37 samples that were diagnosed as papillary or anaplastic carcinomas by cytological examination showed a positive result by gene diagnosis, whereas only 4 (3.7%) of 109 samples that were cytologically diagnosed negative for both carcinomas showed a positive result. Among all of the cases, 50 patients underwent surgery, and a histological diagnosis was consequently made. The sensitivity and specificity of this method were 96.9 and 100%, respectively. A combined examination using both genetic and cytological approaches may contribute to a more precise preoperative diagnosis of papillary and anaplastic carcinomas.
人类乳腺肿瘤中癌胎儿纤连蛋白的分布：组织学切片的免疫荧光研究。
影响因子：
11.2
作者：
Loridon-Rosa,B;Vielh,P;Matsuura,H;Clausen,H;Cuadrado,C;Burtin,P
通讯作者：
Burtin,P