Peptide neurons in the vagus, splanchnic and sciatic nerves.
Peptide neurons in the vagus, splanchnic and sciatic nerves.
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迷走神经、内脏神经和坐骨神经中的肽神经元。
DOI：
10.1111/j.1748-1716.1978.tb06307.x
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发表时间：
1978
期刊：
影响因子：
--
通讯作者：
Saml Sald
中科院分区：
文献类型：
--
作者：
J. Lundberg;Tomas Hökfelt;ÖRan Nilsson;L. Terenius;Jens F. Rehfeld;Robert Elde;Saml Sald
周围神经一般包含运动和感觉纤维。运动神经中的经典递质物质是乙酰胆碱（Loewi，1921）或去甲肾上腺素（Euler，1956），尽管也描述了非胆碱能、非肾上腺素能成分（参见Burnstock，1972）。此外，已经在例如迷走神经提取物中观察到肽，包括P物质（SP）（Euler 1963）、血管活性肠多肽（VIP）（Said和Rosenberg 1976）、胃泌素（GAS）（Uvnas-Wallensten et al. 1977）和生长抑素（SOM）（Uvnas-Wallensten et al. 1978年）。在本通讯3种不同类型的周围神经进行了研究，间接免疫荧光技术使用抗血清的几种肽。用这种方法试图进一步确定这种肽的细胞定位。10只雄性白化病大鼠（B.重量150-200 g）和10只雄性豚鼠（B.重量根据Dahlstrom（1971）所述的技术，对300 - 400 g）进行以下处理。迷走神经在颈部水平结扎，内脏大神经在小脑下水平结扎，坐骨神经在梨状下孔下方1cm结扎。在每种动物的其他5只动物中，将秋水仙碱（20%）或长春碱（1 yo）局部应用于结状神经节和第一骶脊神经节。这些药物和连接技术用于增加肽的水平，因为在未处理的动物中，细胞体和轴突通常含有太低的量，以至于不能用免疫细胞化学观察到。两种处理后24小时，用冰冷的4%多聚甲醛灌注动物，并对神经和神经节进行间接免疫荧光技术处理（参见Coons 1958）。简言之，将低温恒温器切片与针对肽SP、SOM、VIP、GAS和甲硫氨酸-脑啡肽（met-ENK）之一产生的抗血清一起孵育，然后与荧光素-异硫氰酸酯缀合的抗体一起第二次孵育，并在Zeiss荧光显微镜中检查。用过量的相应肽预处理的不同抗血清用作对照血清。关于包括抗血清特征的实验程序的进一步细节，参见Lundberg et al.（1979年）。然而，应该指出的是，GAS抗血清也与胆囊收缩素（CCK）交叉反应。
Peripheral nerves in general contain both motor and sensory fibres. The classical transmitter substances in the motor nerves are either acetylcholine (Loewi, 1921) or noradrenaline (Euler 1956), although a non-cholinergic, non-adrenergic component also has been described (see Burnstock 1972). In addition peptides have been observed eg in extracts of the vagus nerve, including substance P (SP)(Euler 1963), vasoactive intestinal polypeptide (VIP)(Said and Rosenberg 1976), gastrin (GAS)(Uvnas-Wallensten eta/. 1977) and somatostatin (SOM)(Uvnas-Wallensten et a/. 1978). In the present communication 3 different types of peripheral nerves were studied by the indirect immunofluorescence technique using antisera to several peptides. With this approach it was attempted to define further the cellular localization of such peptides.10 male albino rats (b. wt. 150-200 g) and 10 male guinea pigs (b. wt. 300400 g) were subjected to the following treatments according to the techniques described by Dahlstrom (1971). The vagus nerve was ligated at the cervical level, the greater splanchnic nerve at the subdiaphragmatic level and the sciatic nerve one cm below the foramen infrapiriforme. In 5 other animals of each kind colchicine (20%) or vinblastine (1 yo) were applied locally on the nodose ganglion and on the first sacral spinal ganglion. These drugs and the ligation technique were used to increase the levels of the peptides, since in untreated animals the cell bodies and axons often contain too low amounts to be visualized with immunocytochemistry. 24 h after both types of treatment the animals were perfused with icecold 4% paraformaldehyde and the nerves and ganglia were processed for the indirect immunofluorescence technique (see Coons 1958). Briefly, cryostat sections were incubated with an antiserum raised to one of the peptides, SP, SOM, VIP, GAS and methionine-enkephalin (met-ENK) followed by a second incubation with fluorescein-isothiocyanate conjugated antibodies and examined in a Zeiss fluorescence microscope. The different antisera pretreated with an excess of the respective peptide served as control sera. For further details on experimental procedures including characteristics of antisera, see Lundberg et a/.(1979). It should, however, be pointed out that the GAS antiserum also cross-reacts with cholecystokinin (CCK).