Metabolic fate of [14C]-labeled meal protein amino acids in Aedes aegypti mosquitoes
Metabolic fate of [14C]-labeled meal protein amino acids in Aedes aegypti mosquitoes
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DOI：
10.1016/j.jinsphys.2004.02.003
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发表时间：
2004-04-01
影响因子：
2.2
通讯作者：
Wells, MA
中科院分区：
文献类型：
--
作者：
Zhou, GL;Flowers, M;Wells, MA
我们开发了一种方法来跟踪埃及伊蚊在三种不同的成虫营养方案下[C-14]标记的细小裸藻蛋白氨基酸的代谢命运。血粉蛋白氨基酸代谢的定量分析表明，氨基酸的大部分碳要么氧化成CO2，要么作为废物排出体外。在三种不同的成虫营养方案下，氨基酸代谢没有发现显着差异，这表明雌性埃及伊蚊在生殖周期中具有维持代谢稳态的强大能力。在吸血前部分饥饿和/或吸血后饥饿的蚊子中，产卵后母体糖原和脂质的量显着较低。三种不同方案之间的卵脂质或蛋白质含量或产卵数量没有表现出显着差异，这表明在血粉前部分饥饿和/或血粉后饥饿下埃及伊蚊的稳定繁殖力似乎是当前繁殖力和产卵后未来存活率之间权衡的结果。本文描述的方法可应用于有关环境条件对血粉氨基酸利用的影响的广泛问题。 (C) 2004 Elsevier Ltd. 保留所有权利。
We developed a method to follow the metabolic fate of [C-14]-labeled Euglena gracilis protein amino acids in Aedes aegypti mosquitoes under three different adult nutritional regimes. Quantitative analysis of blood meal protein amino acid metabolism showed that most of the carbon of the amino acids was either oxidized to CO2 or excreted as waste. Under the three different adult nutritional regimes, no significant differences in the metabolism of amino acids were found, which indicated that the female A. aegypti mosquitoes possess a substantial capacity of maintaining metabolic homeostasis during a gonotrophic cycle. The amount of maternal glycogen and lipid after egg laying were significantly lower in the mosquitoes that underwent a partial starvation before a blood meal and/or starvation after the blood meal. The content of egg lipid or protein or the number of eggs laid did not show a significant difference among the three different regimes, which indicates that stable fecundity of A. aegypti under the partial starvation before a blood meal and/or starvation after the blood meal seemed to result from a trade-off between current fecundity and future survival after the eggs laid. The methods described in this paper can be applied to a wide range of questions about the effects of environmental conditions on the utilization of blood meal amino acids. (C) 2004 Elsevier Ltd. All rights reserved.