Tetramethylrhodamine is an essential scaffold of azide probe in detecting cellular acrolein
Tetramethylrhodamine is an essential scaffold of azide probe in detecting cellular acrolein
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DOI：
10.1016/j.bmc.2019.04.026
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发表时间：
2019-06-01
影响因子：
3.5
通讯作者：
Tanaka, Katsunori
中科院分区：
文献类型：
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作者：
Pradipta, Ambara R.;Fujii, Motoko;Tanaka, Katsunori
四甲基罗丹明(TAMRA)-苯叠氮化物是一种用于直接在活细胞中检测细胞内丙烯醛的化学探针。在这里，我们证明了TAMRA是探针的最佳荧光团。Tamra-苯叠氮化物被用来揭示在各种乳腺癌细胞中产生高水平的丙烯醛，而不考虑肿瘤亚型。这些发现证实了我们在之前的报告中提出的分析，在该报告中，Tamra-苯叠氮化物被用于标记从乳腺癌患者切除的乳腺癌组织。因为在所有类型的癌细胞中都会产生高水平的丙烯醛，我们认为丙烯醛的检测作为标记癌症组织的一种通用方法可能是有用的。
Tetramethylrhodamine (TAMRA)-phenyl azide is a chemical probe used to detect intracellular acrolein directly in live cells. Herein, we demonstrated that TAMRA is the optimum fluorophore for the probe. TAMRA-phenyl azide was used to reveal that high levels of acrolein are generated in a variety of breast cancer cells, regardless of the tumor subtype. These findings corroborate the analysis presented in our previous report, in which TAMRA-phenyl azide was used to label breast cancer tissues resected from breast cancer patients. Because high levels of acrolein were generated in all cancer cell types, we believe that acrolein detection may be useful as a general method for labeling cancerous tissues.