Mycobacterium tuberculosis detection from oral swabs with Xpert MTB/RIF ULTRA: a pilot study
Mycobacterium tuberculosis detection from oral swabs with Xpert MTB/RIF ULTRA: a pilot study
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DOI：
10.1186/s13104-019-4385-y
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发表时间：
2019-06-20
影响因子：
1.8
通讯作者：
Franke, Molly F.
中科院分区：
文献类型：
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作者：
Mesman, Annelies W.;Calderon, Roger;Franke, Molly F.
目的结核病的诊断检测依赖于痰液中结核分枝杆菌（Mtb）的微生物学检测。对于无法咳痰的患者，例如儿童和艾滋病毒感染者，这给快速诊断和治疗带来了障碍。因此，本研究旨在使用口腔拭子作为通过分子检测检测 Mtb 的替代样本类型。结果在一项试点研究中，我们旨在使用 Xpert MTB/RIF ULTRA 评估通过口腔拭子检测 Mtb 的敏感性。我们招募了来自秘鲁利马的 33 名结核病病例和 30 名对照者，并使用痰液培养物作为参考测试，从口腔拭子中检测出结核分枝杆菌，敏感性为 45%（95% 置信区间 (CI) 29-62%），特异性为 100%（95% CI 89-100%）。我们当前的方案需要优化，但这些结果支持未来探索使用口腔拭子进行结核分枝杆菌检测。
ObjectiveDiagnostic testing for tuberculosis depends on microbiological detection of Mycobacterium tuberculosis (Mtb) in sputum. For patients unable to expectorate sputum, such as children and individuals living with HIV, this poses barriers to rapid diagnosis and treatment initiation. Therefore, this study aimed to use oral swabs as an alternative sample type for Mtb detection via molecular testing.ResultsIn a pilot study, we aimed to evaluate sensitivity of Mtb detection via oral swabs using Xpert MTB/RIF ULTRA. We enrolled 33 TB cases and 30 controls from Lima, Peru, and detected Mtb from oral swabs with a sensitivity of 45% (95% confidence interval (CI) 29-62%) and specificity of 100% (95% CI 89-100%) using liquid culture of sputum as reference test. Our current protocol will need optimization, but these results support future exploration of the use of oral swabs for Mtb detection.