Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis
Initiating a crystallographic analysis of recombinant (S)-2-hydroxypropylphosphonic acid epoxidase from Streptomyces wedmorensis
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DOI：
10.1107/s1744309105012376
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发表时间：
2005-05-01
影响因子：
0.9
通讯作者：
Hunter, WN
中科院分区：
文献类型：
--
作者：
Cameron, S;McLuskey, K;Hunter, WN
环氧乙烷（1 R，2S）-1，2-环氧丙基膦酸（磷霉素）是一种天然产物抗生素，由链霉菌（Streptomyces wedmorensis）通过金属离子依赖性（S）-2-羟丙基膦酸环氧酶产生。这种环氧酶是非常不寻常的，因为它不需要血红素辅基。已克隆了编码该酶的基因fom 4，并建立了该酶的高效重组来源。得到了四方和六方两种不同的晶型。六方结构的对称性与空间群P6（1/5）22和晶胞参数a = 86.44，c = 221.56埃，γ = 120度一致。马修斯系数V-M为2.7埃（3）Da（-1），对应于在具有55%溶剂含量的不对称单元中的两个亚基，每个亚基的重量约为21.4 kDa。这些晶体具有高分辨率，正在寻求实验阶段以确定结构。
The oxirane ( 1R, 2S)-1,2-epoxypropylphosphonic acid ( fosfomycin) is a natural product antibiotic produced in Streptomyces wedmorensis by the metal-iondependent (S)-2-hydroxypropylphosphonic acid epoxidase. This epoxidase is highly unusual since it has no requirement for a haem prosthetic group. The gene encoding the enzyme, fom4, has been cloned and a highly efficient recombinant source of the enzyme established. Two different crystal forms, tetragonal and hexagonal, have been obtained. The hexagonal form displays symmetry consistent with space group P6(1/5)22 and unit-cell parameters a = 86.44, c = 221.56 angstrom, gamma = 120 degrees. The Matthews coefficient, V-M, of 2.7 angstrom(3) Da(-1) corresponds to two subunits, each of approximate weight 21.4 kDa, in the asymmetric unit with 55% solvent content. These crystals diffract to high resolution and experimental phases are being sought to determine the structure.