The expression of zinc finger 804a (ZNF804a) and cyclin-dependent kinase 1 (CDK1) genes is related to the pathogenesis of rheumatoid arthritis
The expression of zinc finger 804a (ZNF804a) and cyclin-dependent kinase 1 (CDK1) genes is related to the pathogenesis of rheumatoid arthritis
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DOI：
10.1080/13813455.2020.1716810
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发表时间：
2020-01-27
影响因子：
3
通讯作者：
Mehanna, Eman T.
中科院分区：
文献类型：
--
作者：
Fattah, Shaimaa A.;Fattah, Maha A. Abdel;Mehanna, Eman T.
背景：ZNF804a和CDK1基因编码参与炎症途径的蛋白质。目的：本研究旨在探讨ZNF804a和CDK1在埃及类风湿关节炎(RA)患者中的表达谱与疾病活动性和严重程度的相关性及其与炎症反应的关系。方法：用定量聚合酶链式反应(qRT-PCR)检测ZNF804a和CDK1基因的表达。对临床和实验室参数进行评估。结果：与正常对照组相比，RA患者ZNF804a表达下调0.177倍，CDK1表达上调3.29倍(p<；.001)。ZNF804a下调与CRP、RF、28个关节疾病活动度评分(DAS-CRP)、肿瘤坏死因子-α呈负相关。类风湿关节炎患者CDK1过度表达与干扰素-1、ACPA相关。结论：ZNF804a和CDK1基因可能通过影响参与类风湿关节炎炎症反应的肿瘤坏死因子-α和干扰素-1而参与类风湿关节炎的发病。
Context: ZNF804a and CDK1 genes code for proteins involved in inflammatory pathways. Objective: This study aimed to investigate the correlation of ZNF804a and CDK1 expression profiles in RA with the activity and the severity of the disease and to assess their association with inflammatory reactions in the Egyptian RA patients. Methods: ZNF804a and CDK1 expression profiles were assessed using quantitative PCR (qRT-PCR). Clinical and laboratory parameters were evaluated. Results: ZNF804a expression was down-regulated by 0.177-fold while CDK1 expression was up-regulated to 3.29-fold in RA patients compared with healthy controls (p < .001). ZNF804a down-regulation was negatively correlated with CRP, RF, disease activity score of 28 joints (DAS) using CRP (DAS-CRP) and TNF-alpha. CDK1 overexpression was correlated with IFN-1 and ACPA in RA patients. Conclusion: ZNF804a and CDK1 genes are implicated in RA pathogenesis due to their influences on TNF-alpha and IFN-1 which contribute to inflammation in RA patients.